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游正博 (John Yu, M.D., Ph.D.)


研究室名稱:林口長庚紀念醫院 幹細胞與轉譯癌症研究所

最高學歷M.D. / Ph.D. 生物物理





研究室現有: 博士後研究員   17

博士班研究生    2

碩士班研究生    0

專任研究助理    6

大學部專題生    0

研究興趣: 幹細胞生物學、造血機制調控、腫瘤生成


本研究室著重於幹細胞與癌症領域的探討,並將成果轉譯至再生醫學與癌症治療上。透過與林口長庚紀念醫院臨床資源之整合,我們亦將發展可用於病人之新穎的臨床診斷方法與治療策略。我們利用創新的技術平台以探討人類胚胎幹細胞或腫瘤幹細胞之獨特標記或調節機制。特別的是,我們利用醣化學/醣蛋白體學、幹細胞生物學、誘發性多功能幹細胞等相關技術並結合基因修飾的平台,進而探究各種幹細胞上不同的醣類/醣蛋白之表現,與其下游因子之調控。藉由我們在人類胚胎幹細胞或腫瘤幹細胞上新發現的標記蛋白,以及針對肺部幹細胞之新的分離技術,我們未來的研究將著重於(1)人類胚胎幹細胞或癌症幹細胞的特殊標記分子(glycosphingolipidsN-linked sialoglycoproteinsphosphoproteines)、下游調節因子、與部份精選過的醣轉化酵素在維持自我更新與分化能力之角色,(2)胚胎幹細胞與癌症間的關係,(3)醣質對於幹細胞之功能與分化能力上之潛在貢獻,(4)肺部幹細胞在支氣管分化中之功能與肺氣腫形成的機制,以及第二型上皮-間質轉化在促進小呼吸道纖維化之相關機制。藉由這些新發現的細胞表面標記分子,目前的研究成果將有助於解決幹細胞與癌症幹細胞之分離與增生,也有助於闡明這些幹細胞與癌症幹細胞的新分子在功能上的角色。另一方面,這些嶄新的技術平台發展除了可促進我們更加了解幹細胞與癌症間的相互關係外,也可促進癌症偵測與針對新醣質/醣蛋白療法的發展。



Selected Recent Relevant Publications (Selected from 113 publications):

1.  Ling, T.Y., Kuo, M.D., … Chen, S.H. and Yu, J. (2006) Identification of pulmonary Oct-4+ stem/progenitor cells and demonstration of their susceptibility to SARS-CoV infection in vitro. PNAS USA, 103: 9530-9535.

2.  Daley, G.Q., Ahrlund-Richter, L., … Yu, J., et al. (2007) The ISSCR guidelines for human embryonic stem cell research. Science, 315:603-604 with 64 pages of supplementary online materials.

3.  Chang, W.W., Lee, C.H., Lee, P., Lin, J., Hsu, C.W., Hung, J.T., Lin, J.J., Yu, J.C., Shao, L.E., Yu, J., Wong, C.H., and Yu, A.L. (2008) Expression of Globo H and SSEA3 in breast cancer stem cells and the involvement of fucosyl transferases 1 & 2 in Globo H synthesis. PNAS USA, 105: 11667-11672.

4.  Kuo, M.W., Wang, S.H., Chang, J.C., …, Li, W.H. and Yu, J. (2009) A novel puf-A gene predicted from evolutionary analysis is involved in the development of eyes and primordial germ-cells. PloS One 4: e4980.

5.  Andrews, P. W., Arias-Diaz, J., Auerbath, J., … Yu, J., et al. (2009) Consensus guidance for banking and supply of human embryonic stem cell lines for research purposes- international cell banking initiative. Stem Cell Rev. 5:301-314.

6.  Lu T.Y., Lu R.M., Liao M.Y., Yu J., Chung C.H., Kao C.F, and Wu H.C. (2010) Epithelial cell adhesion molecule regulation is associated with the maintenance of the undifferentiated phenotype of human embryonic stem cells. J. Biol. Chem., 285: 8719-8732.

7.  Huang, C.J., …, Yu, J.* and Chang Y.C.* (2010) The influence of collagen film nanostructure on pulmonary stem cells and collagen-stromal cell interactions. (*Co-corresponding authors) Biomaterials, 31:8271-8280.

8.  Liang, Y. J.; Kuo, H. H.; Lin, C. H.; Chen, Y. Y.; Yang, B. C.; Cheng, Y. Y.; Yu, A. L.; Khoo, K. H.; Yu, J. (2010) Switching of the core structures of glycosphingolipids from globo- and lacto- to ganglio-series upon human embryonic stem cell differentiation. PNAS USA, 107: 22564-22569.

9.  Cheng, P.Y., Lin, Y.P., Chen, Y.L., Lee, Y.C., Tai, C.C., Wang, Y.T., Chen, Y.J., Kao, C.F., and Yu, J. (2011) Interplay between SIN3A and STAT3 mediates chromatin conformational changes and GFAP expression during cellular differentiation. PLos One, 6: e22018.

10. Liang, Y.J., Yang, B.C., Chen, J.M., Lin, Y.H., Huang, C.L., Cheng, Y.Y., Hsu, C.Y., Khoo, K.H., Shen, C.N., Yu, J. (2011) Changes in glycosphingolipid composition during differentiation of human embryonic stem cells to ectodermal or endodermal lineages. Stem Cells, 29: 1995-2004.

11. Cho, H.C., Lai, C.Y., Shao, L.E., and Yu, J. (2011) Identification of tumorigenic cells in KrasG12D-induced lung adenocarcinoma. Cancer Research, 71:7250-7258.

12. Liao, C.H. and Yu, J. (2012) Human embryonic stem cell differentiation: role of glycosphingolipid structure. Volume 8: p179- 190 “Stem cells and cancer stem cells” (Editor: M. A. Hayat; Springer London) 

13. Chen, Y.H. and Yu, J. (2012) Ectopic expression of Fgf3 leads to aberrant lineage segregation in the mouse parthenote preimplantation embryos. Developmental Dynamics, 241: 1651-1664. 

14. Cho, H.C., Liao, C.H., Yu, A.L. and Yu, J. (2012) Surface markers in stem cells and cancer from the perspective of glycomic analysis. International J. of Biol. Markers, 27(4): 344-352; a review

15. Chang, W.W., Lin, R.J., Yu, J., Chang, W.Y., Fu, C.H., Lai, A. C, Yu, J.C. and Yu, A. L. (2013) The expression and significance of insulin-like growth factor-1 receptor and its pathway on breast cancer stem cells/progenitors. Breast Cancer Research, 15(3):R39

16. Wang, S.H., Wu, Y.T., Kuo, S.C., and Yu, J. (2013) HotLig: a molecular surface-directed approach to scoring protein-ligand interactions. Journal of Chemical Information and Modeling, 53:2181–2195.

17. Wu, T.J., Tzeng, Y.K., Chang, W.W., Cheng, C.A., Kuo, Y., Chien, C.H., Chang, H.C. and Yu, J. (2013) Tracking the engraftment and regenerative capabilities of transplanted lung stem cells using fluorescent nanodiamonds. Nature Nanotechnology, 8:682-689.

18. Lin, J.J., Huang, C.S., Yu, J., Liao, G.S., Lien, H.C., Hung, J.T., Lin, R.J., Chou, F.P., Yeh, K.T. and Yu, A.L. (2014) Malignant phyllodes tumors display mesenchymal stem cell features and ALDH/GD2 identify their tumor stem cells. Breast Cancer Research, 16:R29.

19. Fu, C.H., Lin, R.J., Yu, J., Chang, W.W., Liao, G.S., Chang, W.Y., Tseng, L.M., Tsai, Y.F., Yu, J.C. and Yu, A.L. (2014) Oncogenic role of SH2-containing-5’-inositol phosphatase-2 in breast cancer stem cells. Stem Cells, 32(8): 2048-60.

20. Lin, C.W., Sun, M.S., Liao, M.Y., Chung, C.H., Chi, Y.H., Chiou, L.T., Yu, J., Lou, K.L., Wu, H.C. (2014) Podocalyxin-like 1 promotes invadopodia formation and metastasis through activation of Rac1/Cdc42/cortactin signaling in breast cancer cells. Carcinogenesis, 35: 2425-35.

21. Cheng, J.Y., Wang, S.H., Lin, J., Tsai, Y.C., Yu, J., Wu, J.C., Hung, J.T., Lin, J.J., Wu, Y.Y., Yeh, K.T., Yu, A.L. (2014) Globo-H ceramide shed from cancer cells triggers translin-associated factor X-dependent angiogenesis. Cancer Research, 74:6856-66.

22. Chen, E., Yu, J. (2014) Epigenetic disruptions of histone signatures for the trophectoderm and inner cell mass in mouse parthenogenetic embryos. Stem Cells and Development, 24:550-64.


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