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陳怡原

 

陳怡原 (Yi-Ywan M. Chen)

職稱教授

研究室細菌遺傳研究室

最高學歷:Ph.D.

學校/國家Univ. of Texas HSC at San Antonio / 美國

分機號碼:X3352

電子郵件帳號mchen@mail.cgu.edu.tw

個人網頁網址http://memo.cgu.edu.tw/margaret

是否直接連接至個人網頁

研究室現有: 博士後研究員0

博士班研究生: 1

碩士班研究生: 6

專任研究助理  2

大學部專題生  3

 

研究方向研究室特色

牙菌斑是人體內最常見的生物膜,而牙菌斑細菌也是引起齲齒及牙周病等慢性感染的病源。近年來多項研究顯示多種全身性疾病如冠心病、腦中風及小早產兒的發生機率及嚴重性均與牙周病感染有關,暗示口腔感染會增加全身性感染的機率及危險性。本實驗室的研究興趣在探討環境因子對口腔細菌生理、毒性分子基因表現及口腔疾病發生的影響,並藉此資訊開發可控制口腔感染的藥物療法。為達此目標,我們利用連續性發酵槽及一般batch培養系統進行細菌培養,並以多種生化及分生技術來觀察口腔細菌基因的表現。藉由突變株及報導基因等系統,我們可分析單一基因在特定環境下的表現程度,再以體學技術系統性地觀察每一突變對整體基因表現的影響。我們主要的兩個研究主題是:一,探討唾液鏈球菌尿素酶基因族表現的調控機制及尿素水解對口腔生態平衡、疾病發展的影響;二,探討環境因子對副嗜血鏈球菌及轉糖鏈球菌在生物膜生存型態中的生理調控及致病分子表現。

最近發表論文 (2011-2016)

1. Y. M. Chen*, Y-Y. Chen, J-L. Hung, P-M. Chen, J-S. Chia. 2016. The GlnR Regulon in Streptococcus mutans is differentially regulated by GlnR and PmrA. PLoS ONE 11(7): e0159599.

2. S-C. Huang, and. Y. M. Chen*. 2016. Role of VicRKX and GlnR in pH dependent regulation of the Streptococcus salivarius 57.I urease operon. mSphere 1: e00033-15.

3. C-J. Chang, C-S. Lin, C-C. Lu, J. Martel, Y-F. Ko, D. M. Ojcius, S-F. Tseng, T-R. Wu, Y. M. Chen, J. D. Young and H-C. Lai. 2015. Ganoderma lucidum reduces obesity in mice by modulating the composition of the gut microbiota. 2015, Nat. Commun. 6:7489 (IF=11.470, 3/56, in Multidisciplinary Science)

4. T-W. Chen, R-C. Gan, Y-F. Chang, W-C. Liao,T. H. Wu, C-C. Lee,P-J. Huang, C-Y. Lee, Y. M. Chen, C-H. Chiu, and P. Tang. 2015. Is the whole greater than the sum of its parts? De novo assembly strategies for bacterial genomes based on paired-end sequencing.  BMG Genomics 16:648 (IF=3.986, 26/165 in Biotechnology and Applied Microbiology)

5. S-C. Huang, R. A. Burne and Y. M. Chen*. 2014. The pH-dependent expression of the urease operon in Streptococcus salivarius is mediated by CodY. Appl. Environ. Microbiol. 80: 5386-5393. (IF=3.952, 30/165 in Biotechnology and Applied Microbiology)

6. C-J. Chang, Y. M. Chen, C-C. Lu, C-S. Lin, J. Martel, S-H. Tsai, Y-F. Ko, T-T. Huang, D-M. Ojcius, J-D. Young, H-C. Lai. 2014. Ganoderma lucidum stimulates NK cell cytotoxicity by inducing NKG2D/NCR activation and secretion of perforin and granulysin. Innate Immun. 20:301-311. (IF=2.682, 78/137 in Immunology)

7. K-Y. Huang, Y. M. Chen, Y-K. Fang, W-H. Cheng, C-C. Cheng, Y-C. Chen, T. E. Wu, F-M. Ku, S-C. Chen, R. Lin, P. Tang. 2014. Adaptive responses to glucose restriction enhance cell survival, antioxidant capability, and autophagy of the protozoan parasite Trichomonas vaginalis. Biochemica et Biophysica Acta. 1840: 53-64. (IF=3.848, 89/290 in Biochemistry and Molecular Biology)

8. Y. M. Chen*, H-R. Shieh, and Y-C. Chang. 2013. The expression of the fim operon is crucial for the survival of Streptococcus parasanguinis FW213 within macrophages but not acid tolerance. PLoS ONE 8: e66163 (IF=3.730, 7/56 in Multidisciplinary Sciences)

9. J. Geng, C-H. Chiu, P. Tang, Y. Chen, H-R. Shieh, S. Hu, and Y. M. Chen*. 2012. Complete genome and transcriptomes of Streptococcus parasanguinis FW213: phylogenic relations and potential virulence mechanisms. PLoS ONE 7: e34769 (IF=4.092, 12/85 in Biology)

10. J. A. Garnett, P. J. Simpson, J. Taylor, S. V. Benjamin, C. Tagliaferri, E. Cota, Y. M. Chen, H. Wu and S. Matthew. 2012. Structural insight into the role of Streptococcus parasanguinis Fap1 within oral biofilm formation. Biochem. Biophy. Res. Comm. 417:421-426. (IF=2.595, 154/286 in Biochemistry and Molecular Biology)

11. J. Geng, S-C. Huang, S. Li, S. Hu, Y. M. Chen*. 2011. Complete Genome Sequence of the ureolytic Streptococcus salivarius strain 57.I. J. Bacteriol. 193:5596-5597. (IF=3.726, 25/107 in Microbiology)

12. X. Liang, Y. M. Chen, T. Ruiz and H. Wu. 2011. A new cell surface protein involved in biofilm formation by Streptococcus parasanguinis. Infect. Immun. 79:3239-3248. (IF=4.098, 11/58 in Infectious Diseases)

13. Y. M. Chen*, H-R. Shieh, C-T. Lin, and S-Y. Liang. 2011. Properties and construction of pFW213, a shuttle vector with the oral Streptococcus origin of replication. Appl. Environ. Microbiol. 77:3967-3974. (IF=3.778, 32/160 in Biotechnology and Applied Microbiology)

 

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