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郭敏玲

郭敏玲

職稱:教授

研究室名稱:分子免疫研究室

最高學歷:Ph.D.

學校/國家:Rutgers University / USA

分機號碼:3319/3320

電子郵件帳號:  mingling@mail.cgu.edu.tw

個人網頁網址:https://sites.google.com/site/mlkmolecularimmunology/

研究室現有: 博士後研究員 0

博士班研究生 5

碩士班研究生 4

專任研究助理 3

大學部專題生  2

 

研究方向研究室特色

本實驗室以過敏相關疾病為研究主軸,研究疾病的分子機制及開發新療法。過敏與氣喘疾在已開發國家盛行率愈來愈高,病嚴重影響人民的健康與消耗許多醫療資源,這類疾病是由多個遺傳與環境因子引起體內免疫系統不平衡反應所致,我們的研究重心主要在於深入探討可以調控過敏病的分子與細胞間反應,期許應用於研發新的防治方式。

氣喘病之基因治療:基於氣喘常為第二型T 輔助細胞過度反應所致,嚐試利用病毒載體攜帶抗發炎分子或第一型T 輔助細胞相關之細胞激素基因,經由氣道施予疾病模式小鼠,用以平衡過度活化的第二型T 輔助細胞免疫反應;或是攜帶可干擾第二型T 輔助細胞反應的基因片段,評估其抑制氣喘疾病各項指標的可行性。

藥物或是抗體引發T 調節細胞的發展:近年,如何調節過度活化的免疫反應成為免疫學的熱門課題,我們亦著手研究與表觀遺傳學的基因表達調控相關藥物、或是可改變T 輔助細胞族群活化功能的特殊抗體等,對人類周邊血球中的被激活T 輔助細胞或是氣喘疾病模式小鼠的免疫調節功能。在逐步研究這些藥物如何調控氣喘疾病發生的分子機制過程中,可以進一步發展運用這些藥物或抗體來治療氣喘疾病。

探討可治療氣喘中藥之科學證據:以臨床使用療效佳之方劑如「定喘湯」,利用氣喘動物模式來研究中藥使用之劑量、給藥時機、療效機制及安全性,做為分離出有效成分之基礎;也和中、西醫臨床醫師合作,完成療效評估。在中醫藥科學化方面,則將注重中藥作用於細胞訊號傳遞系統之機制,並搭配以中草藥材中的純化藥物,來探討並分析各藥物對周邊淋巴球細胞活性的調控或是對氣喘疾病模式小鼠的抑制發炎功能。臍帶血淋巴細胞活性研究:比起骨髓,移植臍帶血中造血系統,有材料取得的方便性與排斥性低的優點。所以本實驗室的另外一個研究主題,是和長庚兒童醫院醫師合作,探討以不同細胞激素組合對臍帶血中 T 輔助細胞及自然殺手細胞之複製、死亡及活性的調控,或是評估不同免疫抑制劑對細胞功能的影響,可以對臍帶血移植時的免疫反應,有更多的了解。

 

 

 

近五年發表(從2010起~迄今)

 

*: Correspondence author  : Co-correspondence author  #: equal contribution

 

Pubication                                                

 

2016

Lin T-C, Wu J-Y, Kuo M-L, Ou L-S, Yeh K-W, and Huang J-L. 2016 (Jan 12). Correlation between Disease Activity of Pediatric-onset Systemic Lupus Erythematosus and Level of Vitamin D in Taiwan: A Case-Cohort Study. J. Microbiol. Immunol. Infect. pii: S1684-1182(16)00006-2. IRB: 103-2832B
(DOI:10.1016/j.jmii.2015.12.005)

Cho S, Wu C-J, Yasuda T, Cruz L, Khan A, Lin L-L, Nguyen D, Miller M, Lee H-M, Kuo M-L, Broide DH, Rajewsky K, Rudensky A, Lu L-F. 2016 (Feb 2). MiR-23~27~24 clusters control effector T cell differentiation and function. J. Exp. Med. 213 (2): 235-249.
(DOI: 10.1084/jem.20150990) (NSC 102-2811-B-182-014 and EMRPD1C0131).

Wu C-J, Lu C-H, Chen L-C, Nguyen DT, Huang Y-S, Lin H-H, Lin C-Y, Kuo M-L*. 2016 (May 13).CD4 down regulation and raft dissociation by the non-depleting YTS177 antibody hinder murine T helper cell activities. Biochem. Biophy. Res. Co. 473 (4): 973-979.
(DOI: 10.1016/j.bbrc.2016.04.001 )
(NSC101-2320-B- 182-033,CMRPD1D0411-3and BMRP362)

Chiang N-Y, Chang G-W, Huang Y-S, Peng Y-M, Hsiao C-C, Kuo M-L, Lin H-H. 2016 (June 1). Heparin interacts with adhesion-GPCR GPR56/ADGRG1, reduces receptor shedding, and promotes cell adhesion and motility. J. Cell Sci. 129 (11): 2156-2169.
(
DOI:10.1242/jcs.174458)

Chen J-C, Chan C-C, Wu C-J, Ou L-S, Yu H-Y, Chang H-L, Tseng L-Y, Kuo M-L. Fetal phagocytes take up allergens to initiate Th2 immunity and facilitate allergic airway responses. Am. J. Respir. Crit. Care Med. (accepted) 11 Apr 2016
(DOI: 10.1164/rccm.201508-1703OC)

2015

Yang T-Y, Chiang N-Y, Tseng W-Y, Pan H-L, Peng Y-M, Shen J-J, Wu K-A, Kuo M-L, Chang G-W, Lin H-H*.2015(May). Expression and immunoaffinity purification of recombinant soluble human GPR56 protein for the analysis of GPR56 receptor shedding by ELISA. Protein Expr. Purif. 2015.109: 85- 92.
(DOI: 10.1016/j.pep.2014.11.013.)

Chen J-C, Ou L-S, Yu H-Y, Kuo M-L, Chang P-Y, Chang H-L. 2015 (May). Postnatal donor lymphocytes enhance prenatally-created chimerism at the risk of graft-versus-host disease. Am. J. Transl. Res. 17(5): 941-949.
(DOI: 10.1155/2014/531092. Epub 2014 Jul 20).

(NSC101-2314-B-182-093 (J.C.C.),CMRPG4A0131 (J.C.C.)

Shen C-R, Juang H-H, Chen H-S, Yang C-J, Wu C-J, Lee M-H, Hwang Y-S, Kuo M-L, Chen Y-S, Chen J-K and Liu C-L. 2015 (Nov). The Correlation between Chitin and Acidic Mammalian Chitinase in Animal Models of Allergic Asthma. Int. J. Mol. Sci. 16 (11):27371–27377.
DOI:10.3390/ijms161126033.

(CMRPD1D0032, EMRPD1E1731)

Lin S-J, Huang Y-C, Cheng P-J, Lee P-T, Hsiao H-S, Kuo M-L* . 2015 (Dec). Interleukin-15 enhances the expansion and function of natural killer T cells from adult peripheral and umbilical cord blood. Cytokine. 76(2) :348-355.
(DOI:10.1016/j.cyto.2015.09.015.)

 

2014

Huang W-C , Chan C-C , Wu S-J, Chen L-C, Shen J-J , Kuo M-L, Chen M-C, Liou C-J. 2014 (Jan). Matrine attenuates allergic airway inflammation and eosinophil infiltration by suppressing eotaxin and Th2 cytokines production in asthmatic mice. J. Ethnopharmacol. 151(1): 470-477.
(DOI:
10.1016/j.jep.2013.10.065.)

Chen J-C, Ou L-S, Yu H-Y, Kuo M-L, Chang P-Y and Chang H-L. 2014 (Jul). Recipients with in utero induction of tolerance upregulated MHC class I in the engrafted donor skin. Dis Markers. 2014:531092.
(DOI: 10.1155/2014/531092.)

Lin S-J, Lu C-H, Yan D-C, Lee P-T, Hsiao H-S, Kuo M-L*. 2014 (Oct) Expansion of regulatory T cells from umbilical cord blood and adult peripheral blood CD4+CD25+ T cells. Immunol. Res. 60 (1):105-111.
(DOI: 10.1007/s12026-014-8488-1
.)

Liou C-J, Cheng P-Y, Huang W-C, Chan C-C, Chen M-C Kuo M-L, Shen J-J .2014 (Nov). Oral lovastatin attenuates airway Inflammation and mucus secretion in ovalbumin-induced asthmatic mice. Allergy Asthma Immunol. Res. 6 (6):548-557.
(
DOI: 10.4168/aair.2014.6.6.548.
)

 

2013

Wu C-J, Chen L-C, Huang W-C, Chuang C-L, and Kuo M-L*. 2013Jan. Alleviation of lung inflammatory responses by adeno-associated virus 2/9 carrying CC10 in OVA-sensitized mice. Hu. Gene Ther. 24(1):48-57.
(DOI:10.1089/hum.2012.039.)

Wu C-J, Yang C-Y, Chen Y-H, Chen C-M, Chen L-C, and Kuo M-L*. 2013March. The DNA methylation inhibitor, 5-azacytidine, increases regulatory T cells and alleviates airway inflammation in OVA-sensitized mice. Int. Arch. Allergy Immunol. 160 (4):356-364.
(DOI: 10.1159/000343030)

Wu S-A, Yeh K-W, Lee W-I, Yao T-C, Kuo M-L, Huang B, Huang J-L. 2013 (Jan). Impaired Phagocytosis and Susceptibility to Infection in Pediatric-Onset Systemic Lupus Erythematosus. Lupus. 22(3):279-88.
(DOI: 10.1177/0961203312474704.)

Wu C-J, Chou H-W, Liou C-J, Shen J-J, Wang L-C, and Kuo M-L*. 2013May. Prophylactic Vaccination with Adjuvant Monophosphoryl Lipid A Prevents Th2-Mediated Murine Asthmatic Responses. J. Asthma. 50(4):327-333.
(
DOI:10.3109/02770903.2013.769268)

Lee W-I, Chen, S-H, Huang J-L, Jaing T-H, Chung H-T, Yeh K-W, Chen L-C, Yao T-C, Hsieh M-Y, Lin S-J, Kuo M-L. 2013(May). Identifying patients with neutrophil elastase (ELANE) mutations from patients with a presumptive diagnosis of autoimmune neutropenia. Immunobiol. 218(5):828-8.
(
DOI: 10.1016/j.imbio.2012.09.001.)

Chen J-C, Ou L-S, Yu H-Y, Chang H-L, Chang P-Y, and Kuo M-L. 2013. Jul Allogeneic lymphocytes exerted graft-versus-host rather than tolerogenic effects on preimmune fetuses. J. Surg. Res. 183(1):405-411.
(DOI: 10.1016/j.jss.2012.12.015.)

Chang S-H, Tang P, Lai C-H, M-L Kuo, Wang L-C. 2013 (Sep). Identification and characterization of microRNAs in young adults of Angiostrongylus cantonensis by a deep-sequencing approach. Mem Inst Oswaldo Cruz. 108(6):699-706.
(DOI: 10.1590/0074-0276108062013005.)

Chan C-C, Liou C-J, Xu P-Y, Shen J-J, Kuo M-L, Len W-B, Chang L-E, Huang W-C. 2013. (Nov). Effect of dehydroepiandrosterone on atopic dermatitis-like skin lesions induced by 1-chloro-2,4-dinitrobenzene in mouse. J Dermatol Sci. 72(2):149-57.
(DOI: 10.1016/j.jdermsci.2013.06.015.)

Hsieh C-H, Chen H-C, Chang Y-H, Pang S-T, Kuo M-L, Chuang C-K, Liao S-K. 2013(Nov) Co-existence of epithelioid and fibroblastoid subsets in a sarcomatoid renal carcinoma cell line revealed by clonal studies. Anticancer Res. 33 (11):4875-89.

 

2012

Lin S-J, Cheng P-J, Lin T-Y, Lee P-T, Hsiao H-S, and Kuo M-L*. 2012 (Mar). Effect of influenza A infection on umbilical cord blood natural killer function-regulation with interleukin-15. J. Infect. Dis. 205 (5):745-756.
(
DOI: 10.1093/infdis/jir843)

Chen Y-H #, Kuo M-L #, Cheng P-J, Hsiao H-S, Lee P-T, and Lin S-J. 2012 (Apr). Regulation of CD28 expression on umbilical cord blood and adult peripheral blood CD8+ T cells by interleukin (IL)-15/IL-21. Cytokine. 58(1):40-46.
(DOI:10.1016/j.cyto.2011.12.013)

Lin S-J, Yan D-C, Lee W-I, Kuo M-L, Hsiao H-S, Lee P-Y(T). 2012 (May). Effect of azithromycin on natural killer cell function. Int. Immunopharmacol. 13(1):8-14. (DOI:10.1016/j.intimp.2012.02.013)

Chan C-K, Wu K-H, Lee Y-S, Hwang S-M, Lee M-S, Liao S-K, Cheng E-H, See L-C(史麗珠), Tsai C-N, Kuo M-L, and J-L Huang. 2012 (Jul). The comparison of interleukin-6 associated immunosuppressive effects of human ESCs, fetal-type MSCs, and adult-type MSCs. Transplant. 94(2):132-138.
(Epub: DOI: 10.1097/TP.0b013e31825940a4)

Chen L-C, Tseng H-M, Wu C-J, Kuo M-L, Wu C-J, Gao P-S, Yeh K-W, Yao T-C, Lee W-I, Ou L-S, Huang J-L, and Huang S-K. 2012 (Sep). Evaluation of a common variant of the gene encoding Clara cell 10 kd protein (CC10) as a candidate determinant for asthma severity and steroid responsiveness among Chinese children. J. Asthma. 49 (7):665-672.
( DOI:
10.3109/02770903.2012.697954. Jul 13, 2012)

Wu C-J, Huang W-C, Chen L-C, Shen C-R, and Kuo M-L*. 2012NovPseudotyped adeno-associated virus 2/9-delivered CCL11 shRNA alleviates lung inflammation in an allergen-sensitized mouse model. Hu. Gene Ther. 23 (11): 1156-1165. (DOI:10.1089/hum.2012.012 )

 

2011

Hua M-C, Lai M-W, Kuo M-L, Yao T-C, Huang J-L, Chen S-M. 2011 (Apr). Decreased interleukin-10 secretion in peripheral blood mononuclear cells of children with irritable bowel syndrome. J. Pediatr. Gastr. Nutr 52 (4): 376-381.
(
DOI: 10.1097/MPG.0b013e3181fd9816)

Lin S-J, Lee W-J, Yan D-C, Cheng P-J, and Kuo M-L*. 2011 (May). Azithromycin inhibits IL-5 production of T helper type 2 cells from asthmatic children. Int. Arch. Allergy Immunol. 156 (5):179-186.
(
DOI:10.1159/000322872, May 2011)

Shen J-J, Chiang M-S, Kuo M-L, Hwang T-L, Liou C-J, Huang W-C. 2011 (Jun). Partially purified extract and viscolin from Viscum coloratum attenuate airway inflammation and eosinophil infiltration in ovalbumin-sensitized mice. J. Ethnopharmacol. 135: 646-653
(
DOI: 10.1016/j.jep.2011.03.065)

Tsai Y-C, Yeh K-W, Yao T-C, Huang Y-L, Kuo M-L, Huang J-L. 2011 (Jul). Mannose-binding lectin expression genotype in pediatric-onset systemic lupus erythematosus: associations with susceptibility to renal disease and protection against infections. J. Rheumatol. 38 (7): 1429-1435. (DOI:10.3899/jrheum.100961)

Lee W-I, Huang J-L, Jaing T-H, Shyur S-D, Yang K-D, Chien Y-H, Chiang B-L, Soong W-J, Chiou S-S, Shieh C-C, Lin S-J, Yeh K-W, Chen L-C, Ou L-S, Yao T-C, Lin T-Y, Chiu C-H, Huang Y-C, Wu K-H, Lin C-Y, Yu H-H, Yang Y-H, Yu H-R, Yen H-J, Hsieh M-Y, Kuo M-L, Hwu W-L, Tsai Y-C, Kuo H-C, Lin Y-L, Shih Y-F, Chang K-W. 2011 (Dec). Distribution, clinical features and treatment in Taiwanese patients with symptomatic primary immunodeficiency diseases (PIDs) in a nationwide population-based study during 1985-2010. Immunobiol. 216 (12): 1286-1294.
(
DOI:10.1016/j.imbio.2011.06.002)

 

2010

Liou C-J, Huang W-C, Kuo M-L, Yang R-C, and Shen J-J. 2010 (Oct). Long-term oral administration of Gynostemma pentaphyllum extract attenuates airway inflammation and Th2 cell activities in ovalbumin- sensitized mice. Food Chem. Toxicol. 48(8): 2592-2598.
(DOI:10.1016/j.fct.2010.06.020)

Chen J-C, Kuo M-L, Ou L-S, Chang M-L, Chang P-Y, Muench MO, Shen C-R, Chang H-L, Yu H-Y, and Fu R-H. 2010 (Dec). Characterization of tolerance induction through prenatal marrow transplantation: the requirement for a threshold level of chimerism to establish rather than maintain postnatal skin tolerance. Cell Transpl. 19(12): 1609-1622
(
DOI: 10.3727/096368910X516583)

 

 

Review articles:

 

  2012

Lin S-J, Yan D-C, Lee Y-C, Hsiao H-S, Lee P-T, and Kuo M-L*. 2012 (Feb). Umbilical cord blood immunology: relevance to stem cell transplantation. Clin. Rev. Allergy Immunol. 42(1):45-57.
(DOI: 10.1007/s12016-011-8289-4.)

2011

Lin S-J and Kuo M-L. 2011 (Nov). Cytotoxic function of umbilical cord blood natural killer cells: relevance to adoptive immunotherapy. Pediatr. Hematol. Oncol. 28(8):640-646.
(
DOI:10.3109/08880018.2011.613092)

2008

Lin S-J, Yan D-C, Lee Y-C, and Kuo M-L*. 2008 (Nov). Role of interleukin-15 in umbilical cord blood transplantation. Int. Rev. Immunol. 27 (6):518-531.
(DOI: 10.1080/08830180802317940.)

 

Book chapters:

 

Lin S-J, Lee Y-C, Yan D-C, Kuo M-L. Natural killer cells in allergy. Ch38, pp 511-517, in Lotze M and Thomson A, eds. Natural Killer Cells, Basic Science and Clinical Application, Elsevier, San Diego, CA, USA. 2009.

Lin S-J, Lee P-T, Kuo M-L. Cytokine activation of natural killer cells. Ch19. pp  in Cancer Vaccines: Methods and Protocols, Methods in Molecular Biology, Vol 1139. Springer Science+Business Media, New York, NY, USA. DOI:10.1007/978-1-4939-0345-0_19.

 

Perspectives or comments:

 

Kuo M-L*. 2008 (Aug). Attempts to bridge the gap between scientific mechanism-based medicine and Chinese herbal extract treatment for asthma. Pediatr. Neonatol. 49(4): 101-102. (editorial comments, not SCI)
(DOI: 10.1016/S1875-9572(08)60022-4.)

Lin H-H, Kuo M-L. 2012 (Mar). The 2011 Nobel Prize in Physiology or Medicine. Chang Gung Med. J. 35(2):93-95.

 

2016/06/01更新

 

 

  

 

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