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Graduate Institute of Biomedical Sciences

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王子堅

王子堅 (Tzu-Chien Wang)

AppointmentProfessor

LabMolecular Genetics Laboratory

EducationPh.D.

University/NationTexas University, USA

Tel: (03)2118800 ext.5122

E-mailtcvwg@mail.cgu.edu.tw

Research website

Research interests:

 A. Regulation of Telomerase in Human Cells. Telomerase is a ribonucleoprotein that synthesizes telomeric DNA onto chromosomal ends. Telomerase activity has been identified in most immortal cell lines and in cancer cells, but not in normal somatic cells, linking this enzyme to cell immortality and cancer. The long-term objective of this laboratory is to understand how telomerase is regulated in human cells and its role in carcinogenesis and cellular aging. The ongoing research projects include: (i) regulation of telomerase activity in cancer cells and normal somatic cells in culture, (ii) identification of authentic repressors for hTERT expression in normal human cells, and (iii) the potential use of telomerase expression in the clinical diagnosis and/or therapeutics of cancers.

B. Role of hnRNP A/Bs in the regulation of telomerase and telomere maintenance.
Functional telomeres protect chromosomal ends from DNA degradation and unwanted recombination, and are known to play an important role in cellular senescence. While telomerase and telomere-associated proteins are known to involve in the maintenance of functional telomeres, little is known if heterogeneous ribonucleoproteins (hnRNPs) may function in telomere maintenance. We have shown recently that hnRNPs are the most abundant proteins in the nuclear extracts that bound specifically to G-strand telomeric DNA. The findings that many of these hnRNPs inhibits telomerase in vitro and the fact that hnRNPs are integral components of the nuclear matrix suggest that hnRNPs can participate in the control of telomerase activity and in telomere maintenance in vivo. Currently, we are investigating the biological role of hnRNP A/Bs in the control of telomerase activity and telomere length maintenance in human cells.

 

Publications:

1、Huang, P.R., Yeh, Y.M., Pao, C.C., Chen, C.Y., Wang, T. V. *(2012) N-(1-Pyrenyl) maleimide inhibits telomerase activity in a cell free system Molecular Biology Reports 39:8899-8905(Impact Factor=1.698, Rank=226/289, Categories:Biochemistry & Molecular Biology)

2Dong, Y.M. ; Chien, K.Y. ; Chen, J.T. ; Lin, S.J. ; Wang, T.C.V. ; Yu, J.S.* (2013) Site-specific separation and detection of phosphopeptide isomers with pH-mediated stacking capillary electrophoresis-electrospray ionization-tandem mass spectrometry. Journal of Separation Science 36:1582-1589.(Impact Factor=2.741, Rank=21/75, Categories:Chemistry, Analytical )

3. Yeh, Y.M., Huang, K.Y., Gan, R.R., Huang, H.D., Wang, T.C.V., Tang, P.* (2013)

 Phosphoproteome Profiling of the sexually transmitted pathogen Trichomonas vaginalis

ournal of Microbiology Immunology and Infection 46:366-373. (Impact Factor=2.955,

Rank=68/151, Categories:Immunology)

4. Chen, C.Y. ; Yan, S.C. ; Lee, K.H. ; Yang, X.M. ; Wei, L.Y. ; Chow, L.P. ; Wang, T.C.V. ; Hong, T.M. ; Lin, J.C. ; Kuan, C. ; Yang, P.C.* (2014) The Antitumor Agent PBT-1 Directly Targets HSP90 and hnRNP A2/B1 and Inhibits Lung Adenocarcinoma Growth and Metastasis. Journal of Medicinal Chemistry 57:677-685.( Impact Factor=5.589, Rank=3/59, Categories:Chemistry, Medicinal)

5. Yeh, Y.M.; chen, .CY. ; Huang, P.R. ; Hsu, C.W.; Wu,C.C .; Wang, T.C.V. * (2014) Proteomic analyses of genes regulated by heterogeneous nuclear ribonucleoproteins A/Bs in Jurkat cells Proteomics 14:1357-1366. (Impact Factor=4.079, Rank=13/77, Categories: Biochemical Research Methods)

6. Huang, P.R., Hung, S.C., Pao, C.C., Wang, T.C.V. * (2015) N-(1-Pyrenyl)Maleimide Induces Bak Oligomerization and Mitochondrial Dysfunction in Jurkat Cells.BioMed Research International 2015,798489.(Impact Factor=2.134, Rank=81/161, Categories: Biotechnology & Applied Microbiology)

7. Chen,C.Y., Yu, Z.Y., Chuang, Y. S., Huang, R. M., Wang, T. C.* (2015) Sulforaphane attenuates EGFR signaling in NSCLC cells. Journal of Biomedical Science 22:1-9 (Impact Factor=2.935, Rank=45/124, Categories:Medicine, Research& Experimental)

8. Chi-Yuan Chen, Chia-Ing Jan, Wen-Chieh Pi, Wen-Lung Wang, Pan-Chyr Yang, Tong-Hong Wang, Rotem Karni, Tzu-Chien V. Wang* (2016) Heterogeneous nuclear ribonucleoproteins A1 and A2 modulate expression of Tid1 isoforms and EGFR signaling in non-small cell lung cancer. Oncotarget 7: 16760-16772.

   (Impact Factor=5.008, Rank=36/213, Categories:Oncology)

 

 

 

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