長庚大學 生物醫學研究所



皮海薇 (Haiwei Pi)


職稱:副教授 研究室:發育及幹細胞生物學研究室
最高學歷:博士 學校/國家:State University of New York at Stony Brook/ USA
分機號碼:3361 電子郵件帳號:haiwei@mail.cgu.edu.tw

博士後研究員 1
碩士班研究生 3
專任研究助理 1
大學部專題生 3

個人網頁: http://haiwei.tw

Research interest:

      My laboratory is interested in studying how cells decide to differentiate into specialized cells with distinct functions. This process, call cell fate determination, is critical for embryonic development and adult tissue homeostasis. We study cell fate determination in the context of whole animal, the powerful genetic model organism fruit fly Drosophila melanogaster, combined with molecular, cellular and confocal microscopic approaches.

      There are two major topics in the lab: (1) the roles of nuclear actin in differentiation of sensory organ precursors (SOPs), the neural precursors of Drosophila peripheral sensory organs. Actin, the major components of cytoskeleton, has recently been demonstrated to play important roles in several basic nuclear processes such as RNA polymerase-mediated transcription, chromatin remodeling, and RNA processing. By combining biochemical and molecular analyses in culture cells as well as genetic and functional analysis in Drosophila, we show that nuclear actin is required for neural precursor differentiation by acting as transcriptional co-factor of bHLH neurogeneic proteins Achaetae and Scute. In the future, we would like to study how the specific association between nuclear actin and Achaetae/Scute is achieved, and how nuclear actin levels might be regulated during neurogenesis and development.

      The second topic in the lab is to study the roles of protein degradation and cell signaling in self-renewal and differentiation of male germline stem cells (GSCs), the adult stem cells that produce gametes throughout the reproductive life of male animals. Our study has shown that BMP signaling, the key regulators of GSC self-renewal, is downregulated as male gonads develop. The downregulation is mediated by the ubiquitin E3 ligase Smurf to control the numbers of GSCs as well as the transit-amplifying cells, the differentiating daughter cells of the GSCs. In the future, we will focus on the regulation of GSC self-renewal and differentiation as well as BMP signaling in GSCs by the stem cell microenvironment.


      我們主要用果蠅這個模式生物來研究在活體中,基因如何調控細胞細胞命運決定及分化。實驗室中有兩個主要方向:(1)探討神經母細胞的形成過程中,原神經蛋白如何活化下游基因來促進母細胞分化。我們目前著重於探討核內肌動蛋白和原神經蛋白之間的關係。(2)成體幹細胞 (adult stem cell) 自我新生的調控在 維持組織恆定中扮演 重要功能 。自我新生能力太強時, 細胞分化會被抑制。 反之, 過早進入細胞分化會降低幹細胞的自我新生。我們實驗室最近的研究在探討成體中的配子幹細胞如何受到BMP 或其它訊息傳導路徑的影響來維持在自我新生及細胞分化中的協調。




2012/8/1~2015/7/31 探討COP9複雜亞單位對於果蠅蛻皮激素訊息傳遞鏈的抑制機轉

2015/8/1-2018/7/31 Arp6 及 H2Av 在果蠅外感覺器官分佈及發育的功能

2018/8/1-2019/7/31 bHLH 原神經蛋白在神經母細胞中對核醣體的生化合成的調控


2013/1/1~2015/12/31 老化果蠅雄性生質幹細胞系統中,smurf扮演抑制細胞過度增生的角色

2016/6/1-2019/5/31 在雄性果蠅性腺中,JNK訊息傳遞鍊調節囊幹細胞恆定的分子機制



學年度 姓名 獲獎項目
100 蕭韻凌
100 謝昌哲
100 黃意霖
100 廖唯甯
101 蕭韻凌
101 黃亦君
2013年Asia-Pacific Drosophila Research Conference (韓國漢城)獲得傑出壁報獎
102 黃亦君

Publications (* Corresponding author):  

1. Yi Chieh Chang, Hsin Tu, Jing-Yi Chen, Ching-Chin Chang, Shu Yuan Yang, Haiwei Pi* (2019).

    Reproduction disrupts stem cell homeostasis in testes of aged male Drosophila via an induced microenvironment. 

    PLOS Genetics (accepted)

2. Yang SY, Chang YC, Wan YH, Whitworth C, Baxter EM, Primus S, Pi H, Van Doren M. (2017). Control of a Novel Spermatocyte-Promoting Factor by the Male Germline Sex Determination Factor PHF7 of Drosophila melanogaster.. GENETICS, 206(4),1939-1949.

3. Chien-Hsiang Wang, Yi-Chun Huang, Pei-Yi Chen, Ying-Ju Cheng, Hsiu-Hua Kao, Haiwei Pi , Cheng-Ting Chien (2017). USP5/Leon deubiquitinase confines postsynaptic growth by maintaining ubiquitin homeostasis through Ubiquilin . eLife, 10(6),e26886.

4. Shih-Han Kao, Chen-Yuan Tseng, Chih-Ling Wan, Yu-Han Su, Chang-Che Hsieh, Haiwei Pi, and Hwei-Jan Hsu1 (2015). Aging and insulin signaling differentially control normal and tumorous germline stem cells. Aging Cell, 14(1): 25–34.

5. Huang YC, Lu YN, Wu JT, Chien CT*, Pi H*. (2014) The COP9 Signalosome Converts Temporal Hormone Signaling to Spatia Restriction on Neural Competence. PLoS Genetics 10(11):1004760.

6. Hsiao YL, Chen YJ, Chang YJ, Yeh HF, Huang YC and Pi H*. (2014) Proneural proteins Achaete and Scute associate with nuclear actin to promote external sensory organ formation. Journal of Cell Science 127:182-90.

7. Chang YJ†, Pi H†*, Hsieh CC, Fuller MT. (2013) Smurf-mediated differential proteolysis generates dynamic BMP signaling in germline stem cells during Drosophila testis development. Developmental Biology 383(1):106-20. (†These two authors contributed equally to this work).

8. Yang WK, Peng YH, Li H, Lin HC, Lin YC, Lai TT, Suo H, Wang CH, Lin WH, Ou CY, Zhou X, Pi H, Chang HC, Chien CT.* (2011) Nak regulates localization of clathrin sites in higher-order dendrites to promote local dendrite growth. Neuron 72, 285-299.

9. Pi H*, Huang YC, Chen C, Lin CD, Yeh HF, Pai LM. (2011) Identification of 11-amino acid peptides that disrupt Notch-mediated processes in DrosophilaJournal of Biomedical Science 18, 42. (These two authors contributed equally to this work).

10.Wu JT, Lin WH, Chen WY, Huang YC, Tang CY, Ho MS., Pi H, Chien CT*. (2011) CSN-mediated deneddylation differentially modulates Ci155 proteolysis to promote Hedgehog signalling responses. Nature Communications2,182.

11. Pi H, Lee LW, Lo SJ*. (2009) New Insights into Polycistronic Transcripts in Eukaryotes. Chang Gung Med J. 32, 494-8.

12. Chang PJ, Hsiao YL, Tien AC, Li YJ, Pi H*. (2008) Negative feedback regulation of proneural proteins controls the timing of neural precursor division. Development 135, 3021-30. (Recommended by Dr. Patricia Simpson in  Faculty of 1000).

13. Ho MS, Ou CY, Chan Y, Chien CT.*, Pi H*. (2008) The utility F-box for protein destruction. Cellular and Molecular Life Science 65, 1977-2000.

14. Pi H and Chien CT.* (2007) Getting the edge: neural precursor selection. Journal of Biomedical Science 14, 467-473.

15. Pi H, Huang SK, Tang CY, Sun H, Chien CT*. (2004) phyllopod is a target gene  of proneural protein in Drosophilaexternal sensory organ development. PNAS. 101, 8378-83.

16. Chang CW, Pi H, Chien CT, Hsu CP*. (2003) Network modeling of Drosophila external sensory organ precursor formation: The role of recently studied genes. Journal of Genetics and Molecular Biology. (Taiwan) 14, 243-251

17. Ou CY, Pi H, Chien CT*. (2003) Control of protein degradation by E3 ubiquitin ligases in Drosophila eye development. Trends in Genetics 19, 353-414.

18. Pi H, Wu HJ, Chien CT*. (2001) A dual function of phyllopod in Drosophila external sensory organ development: cell fate specification of sensory organ precursor and its progeny. Development 128, 2699-2710.

19. Pi H, Chien CT, Fields S*. (1997) Transcriptional activation upon pheromone stimulation mediated by a small domain of Saccharomyces cerevisiae Ste12p. Molecular and Cellular Biology 17, 6410-6418.


  • 友善列印
  • 新增到收藏夾
  • 分享