鄧致剛 (Petrus Tang)

最高學歷：Ph.D.

學校/國家：劍橋大學/英國

分機號碼：03-2118800#5136

電子郵件帳號： petang@mail.cgu.edu.tw

個人網頁網址：http://petang.cgu.edu.tw

研究室現有： 博士後研究員1人

博士班研究生 人

碩士班研究生1人

專任研究助理3人

大學部專題生 人

研究室特色：

分子調控暨生物資訊研究室之主要研究目標為以單細胞生物陰道鞭毛蟲作實驗材料，探討細胞壽命延長及化的調控機轉。研究特色是利用生物資訊整合各種經由高通量分析平臺所產生之數據對基因體、轉譯體，蛋白質體作為基礎，並從此等資料中進行探磡，發掘可能調命單細胞原生動物壽命延長及化的機轉。

研究方向：陰道鞭毛蟲微小蛋白調控缺鐵反應之研究

微小蛋白質是由少於一百個胺基酸所組成的蛋白質，最近的研究顯示這群蛋白質參與許多細胞內反應，如腫瘤生成、壓力反應及訊息傳遞等。然而，因為傳統的基因註解方式將一百個胺基酸的長度作為一個閥值，將轉譯出短於一百個胺基酸的開放閱讀框排除，導致微小蛋白一直是被忽略的一群。

基於近年發展成熟的次世代定序技術，其應用廣泛，不僅讓微小蛋白質得已被鑑定出來，更藉此能獲得更全面的基因表現資訊。然而截至目前為止，微小蛋白質尚未在任何原蟲中被發現。本研究室將利用陰道鞭毛蟲作為一個模式生物，鑑定並探討微小蛋白質在缺鐵壓力下所扮演的角色。陰道鞭毛蟲是造成人類非病毒性、盛行率最高的性接觸傳染病陰道鞭毛蟲症的病原體。鐵離子對於調控陰道鞭毛蟲細胞內能量代謝、氧化還原平衡及氫化酶體質量等相當重要，因此陰道鞭毛蟲如何在缺鐵情況下生存是相當重要的課題。經由全基體開放閱讀框、第二代/第三代高通量定序及蛋白質體學整合性生物資訊分析，已初步確認在陰道鞭毛蟲的轉錄體及蛋白體中可發現新的微小蛋白。這初步研究結果開啓了一個全新的研域。因此未來計畫的目標為研究陰道鞭毛蟲的微小蛋白質在缺鐵情況的功能性角色，並且建立一套針對微小蛋白質的實驗及分析平台。

最近五年所發表論文：

1.     Cheng WH, Huang KY, Ong SC, Ku FM, Huang PJ, Lee CC, Yeh YM, Lin R, Chiu CH. Tang P. (2020, Sep). Protein cysteine S-nitrosylation provides reducing power by enhancing lactate dehydrogenase activity in Trichomonas vaginalis under iron deficiency. Parasites & Vectors, 13(1): 477-491. (SCI, 3/23, TROPICAL MEDICINE). MOST 107-2320-B-182-021-MY3. 本人為通訊作者.

2.     Lin HC, Chu LJ, Huang PJ, Cheng WH, Zheng YH, Huang CY, Hong SW, Chen LC, Lin HA, Wang JY, Chen RM, Lin WN, Tang P, Huang KY. (2020, Jun). Proteomic signatures of metronidazole-resistant Trichomonas vaginalis reveal novel proteins associated with drug resistance. Parasites & Vectors, 13(1):274. (SCI, 3/23, TROPICAL MEDICINE).

3.     Huang KY, Ong SC, Wu CC, Lin HC, Fang YK, Cheng WH, Huang PJ, Chiu CH, Tang P. (2019, Aug). Metabolic reprogramming of hydrogenosomal amino acids in Trichomonas vaginalis under glucose restriction. J Microbiol Immunol Infect., 52(4):630-637. (SCI, 33/93, INFECTIOUS DISEASES). MOST 105-

2320-B-016-019-MY2. 本人為通訊作者.

4.     Lee CC, Chen WH, Huang PJ, Yeh YM, Chen SY, Chiu CH, Tang P. (2019, Jul). Pathogenic Protist Transmembranome database (PPTdb): A web-based platform for searching for and analysis of protist transmembrane proteins. BMC Bioinformatics 20(S13):382. (SCI, 9/59, MATHEMATICAL & COMPUTATIONAL BIOLOGY). MOST 107-2320-B-182-021-MY3. 本人為通訊作者.

5.     Huang KY, Chen RM, Lin HC, Lin HA, Lin WN, HuangPJ, Chiu CH, Tang P (2019, Apr). Potential role of autophagy in proteolysis in Trichomonas vaginalis. J Microbiol Immunol Infect., 52(2), 336-344. (SCI, 33/93, INFECTIOUS DISEASES). MOST 105-2320-B-016-019. 本人為通訊作者.

6.     Huang PJ, Lee CC, Chiu LY, Huang KY, Yeh YM, Yang CY, Chiu CH, Tang P. (2018, May). VAReporter: Variant reporter for cancer research of massive parallel sequencing. BMC Genomics 19(S2):86, 19(S2):86. (SCI, 43/156, BIOTECHNOLOGY & APPLIED MICROBIOLOGY). 本人為通訊作者.

7.     Huang PJ, Chiu LY, Lee CC, Yeh YM, Huang KY, Chiu CH, Tang P (2018, Jan). mSignatureDB: a database for deciphering mutational signatures in human cancers. Nucleic Acids Research, 46(D1), D964-D970. (SCI, IF 11.5201, 15/297,

BIOCHEMISTRY & MOLECULAR BIOLOGY). 本人為通訊作者.

8.     Chen TW, Gan RC, Fang YK, Chien KY, Liao WC, Chen CC, Wu TH, Chang IY, Yang C, Huang PJ, Yeh YM, Chiu CH, Huang TW, Tang P (2017, Sep). FunctionAnnotator, a versatile and efficient web tool for non-model organism annotation. Scientific Reports, 7(1):10430. (SCI, IF 4.259, 10/64, MULTIDISCIPLINARY SCIENCES). 本人為通訊作者.

9.     Cheng WH, Huang KY, Huang PJ, Lee CC, Yeh YH, Ku FM, Lin R, Cheng ML, Chiu CH Tang P (2017, Sep). γ-Carboxymuconolactone decarboxylase: a novel cell cycle-related basal body protein in the early branching eukaryote Trichomonas vaginalis. Parasites & Vectors, 10(1):443. (SCI, IF 3.163, 2/20, TROPICAL MEDICINE). MOST 104-2320-B-182-025-MY3. 本人為通訊作者.

10.   Sablok G, Chen TW, Lee CC, Varotto C, Gan RC, Nayak KC, Huang PJ, Tang P (2017, Jun). ChloroMitoCU: Codon patterns across organelle genomes for functional genomics and evolutionary applications.. DNA Research, 24(3):327- 332. (SCI, IF 5.415, 23/171, GENETICS & HEREDITY). 本人為通訊作者.

11.   Gan RC, Chen TW, Wu TH, Huang PJ, Lee CC, Yeh YM, Chiu CH, Huang HD, Tang P. (2016, Dec). PARRoT- A homology-based strategy to quantify and compare RNA-Sequencing from non-model organisms. BMC Bioinformatics, 17(S19):513. (SCI, IF 2.435, 10/56, MATHEMATICAL & COMPUTATIONAL BIOLOGY). 本人為通訊作者.

12.   Fang YK, Chien KY, Huang KY, Cheng WH, Ku FM, Lin R, Chen TW, Huang PJ, Chiu CH, Tang P (2016, Nov). Responding to a zoonotic emergency with multi-omics research: Pentatrichomonas hominis hydrogenosomal protein characterization with use of RNA sequencing and proteomics. OMICS: A Journal of Integrative Biology, 20(11): 662-669. (SCI, IF 2.896, 27/254, PHARMACOLOGY & PHARMACY). 本人為通訊作者.

13.   Huang KY, Ku FM, Cheng WH, Lee CC, Huang PJ, Chu LCJ, Cheng CC, Fang YK, Wu HH, Tang P (2015, Nov). Novel insights into the molecular events linking to cell death induced by tetracycline in the amitochondriate protozoan Trichomonas vaginalis. Antimicrob. Agents Chemother, 59(11):6891-903. (SCI, IF 4.476, 27/254, PHARMACOLOGY & PHARMACY). MOST 101-2320-B- 182-025-MY3. 本人為通訊作者.

14.   Chen TW, Gan RC, Chang YF, Liao WC, Wu TH, Lee CC, Huang PJ, Lee CY, Chen YYM, Chiu CH, Tang P (2015, Aug). Is the whole greater than the sum of its parts? De novo assembly strategies for bacterial genomes based on paired-end sequencing. BMC Genomics, 16:648. (SCI, IF 3.986, 26/162, BIOTECHNOLOGY & APPLIED MICROBIOLOGY). 本人為通訊作者.

15.   Chen WH, Huang KY, Huang PJ, Hsu JH, Fang YK, Chiu CH, Tang P (2015,

Aug). Nitric oxide maintains cell survival of Trichomonas vaginalis upon iron depletion. Parasites & Vectors, 8, 393. (SCI, IF 3.163, 2/20, TROPICAL MEDICINE). MOST 104-2320-B-182-025-MY3. 本人為通訊作者.

16.   Huang PJ, Lee CC, Yeh YM, Huang KY, Gan RC, Chen TW, Lee CY, Yang ST, Liao CS, Liu H, Tang P. (2015, Feb). Vanno: A Visualization-aided Variant Annotation Tool. Human Mutation, 36(2):167-74. (SCI, IF 5.144, 26/167;

GENETICS & HEREDITY). 本人為通訊作者.

17.   Huang PJ, Lee CC, Tan BCM, Yeh YM, Chu JLC, Chen TW, Chang KP, Gan RC, Liu H, Tang P (2015, Jan). CMPD: cancer mutant proteome database. Nucleic Acids Research, 43:D849-55. (SCI, IF 11.561, 10/292, BIOCHEMISTRY & MOLECULAR BIOLOGY). MOST 103-2632-B-182-001. 本人為通訊作者.