莊宏亨

莊宏亨 (Horng-Heng Juang)

職稱：教授

研究室：分子內分泌暨細胞代謝

最高學歷：Ph.D.

學校/國家：馬里蘭大學巴爾的摩分校/美國

分機號碼：(03)-2118800 X5071

電子郵件帳號：hhj143@mail.cgu.edu.tw

個人網頁網址：

是否直接連接至個人網頁：是

研究室現有：
博士後研究員 0 人

博士班研究生： 0 人

碩士班研究生： 3 人

專任研究助理： 2 人

大學部專題生： 1 人

 

個人經歷： ：

中文姓名：莊宏亨

英文姓名：Horng-Heng Juang

出生地：台北市

聯絡住址：桃園縣龜山鄉文化一路259號; 長庚大學解剖科

聯絡電話：(03) 2118800 X5071

傳真機號碼：(03) 2118112

學經歷：

長庚大學解剖科教授 (2012/ 8 -)

長庚大學解剖科副教授 (1996/8 – 2012/7)

長庚大學解剖科助理教授 (1994/10 – 1996/7)

馬里蘭大學巴爾的摩分校 (University of Maryland at Baltimore) 生理學博士

愛我華州立大學 (Iowa State University) 生殖生理學碩士

 

教授課程：大體解剖學、組織學、內分泌特論，書報討論

研究主題：

前列腺癌之發生率及致死率會因人種及區域而不同，雖然一般皆認為在亞洲之中國人得到前列腺癌之機率遠較西方人低。利用衛生署過去二十年來，台灣地區前列腺癌通報之資料作一對人種、地區、及年齡之統計分析，我們發現雖然前列腺癌之發生率及致死率在台灣地區與歐美國家比較仍相當低，但其之發生率及致死率卻逐年增加。在過去二十年內，台灣地區因前列腺癌致死之人口比率已增加一倍。此統計結果證實除了受老化人口急劇增加之因素外，應仍受其它未知因素(如微量離子,遺傳基因等)影響。膀胱癌是最常好發於泌尿道的癌症之一，在全世界統計曾指出膀胱癌亦是好發癌症總排名的第五位。根據台灣的統計膀胱癌亦是第四位好發的泌尿道腫瘤。因為膀胱癌的症狀不太明顯，僅以血尿為主，因此膀胱癌的診斷不易，所以找尋一種敏感度及專一性高的診斷工具是我們極需努力的方向。故本實驗室主要是探討關於前列腺、癌膀胱癌與乳癌演變時之特定oncogenes, tumor suppress genes, metastasis induce genes之基因調控機制，利用 in vitro細胞株與 in vivo動物實驗來證實藥物與荷爾蒙在前列腺癌、膀胱癌與乳癌對特定基因之作用與細胞癌化演變所扮演之功能，並嘗試利用抗癌藥物或傳統中草藥來控前列腺癌、膀胱癌與乳癌之演變。

 

主要研究目標：

  1. 生殖生理 (Reproduction physiology)
  2. 分子內分泌 (Molecular endocrinology)
  3. 細胞代謝 (Cellular metabolism)
  4. 基因調控 (Gene regulation)
  5. 前列腺癌 (Prostate cancer)
  6. 膀胱癌 (Bladder cancer)
  7. 乳癌(Breast cancer)

 

近年研究主題：

  1. 調控檸檬酸代謝之基因轉錄於前列腺

  2. 微量離子對前列腺與膀胱癌之影響

  3. 雄性素受體與甲狀腺素受體對前列腺之調控

  4. 調控前列腺癌轉移之機制與探討膀胱癌之腫瘤標的基因

    A. 探討APRO家族基因之調控對前列腺癌之影響

    B. 攝護腺Kallikrein 基因表達與前列腺癌之相關性病探討齊調節機制

    C. 探討葡萄糖及膽固醇調控粒線體烏頭酶基因之機制

    D. 探討影響細胞生長的基因－CD82在前列腺中的表現機制

    E. 評估Uroplakin與 MT家族基因之表達與調控於膀胱癌

    F. 探討PDEF之功能與調控機制於前列腺與膀胱

    G. 探討影響細胞NDRG family genes在前列腺與膀胱中的表現機制

    H. 探討影響細胞PSCA，BTG2, IL6與GDF15在前列腺與膀胱中的表現機制

  5. 維他命D對乳癌、肝癌與膽道癌之機制

  6. 探討WISP1 之功能與調控機制

研究室特色與研究方法：

實驗室位於醫學大樓之八樓生物醫學共同研究室。實驗室雖不大但精緻且活力十足。實驗室工作主要是以前列腺癌 (prostate cancer) 與膀胱癌 (bladder cancer) 細胞株培養為主體，並以前列腺癌與膀胱癌細胞注射於 nude mice 做為動物實驗(Xenograft animal study)。研究方法主要利用 clone 基因之cDNA及5’-flankling region來探討基因調控之機制。利用 immunoblotting assay， ELISA，3H-incorporation assay， matrigel invasion assay，soft agar cloning assay， xanograft animal study，transient gene expression assay 與其它分生或生化方法來探討藥物，賀爾蒙處理後特定基因之酵素活性，蛋白質，RNA 量。並利用電腦分析，質體移殖及冷光儀測定 reporter activity 來探討基因轉譯之調節。利用 Adenovirus 及 Retrovirus 來攜帶基因進入細胞株或動物體內來探討基因之特定功能。

成果與著作：

1. Juang HH, Musah AI, Schwabe C, Anderson LL. 1989. Effect of relaxin and antirelaxin serum on porcine sperm motility. Animal Reproduction Science 20:21-29.

2. Juang HH, Musah AI, Schwabe C, Anderson LL. 1990. Immunoactive relaxin in boar seminal plasma and its correlation with sperm motility. Animal Reproduction Science 22:47-53.

3. Juang HH, Musah AI, Anderson LL. 1990. Ethylendiamine-tetracacetic acid (EDTA) and caffeine are antagonistic to anti-relaxin serum inhibition of porcine sperm motiligy. Animal Reproduction Science 22:253-260.

4. Kapcala LP, Juang HH, Weng CF. 1990. Corticotropin-releasing hormone and dexamethasone do not alter secretion of immunoreactive-beta-endorphin from dissociated fetal hypothalamic cell culture. Brain Research 532:76-81.

5. Kapcala LP, Weng CF, Juang HH. 1992. Protein kinase C activation stimulates

beta-endorphin secretion from hypothalamic cells. Brain Research Bulletin 29:553-557.

6. Juang HH, Costello L, Franklin R. 1995. Androgen modulation of multiple start sites of the mitochondrial aspartate amino-transferase gene in rat prostate. Journal of Biology Chemistry 270:12629-12634.

7. Franklin R, Juang HH, Zou J, Costello L. 1995. Regulation of citrate metabolism by androgen in LNCaP human prostate carcinoma cell line. Endocrine 3:603-607.

8. Juang HH, Musah AI, Schwabe C, Ford, JJ, Anderson, LL. 1996. Relaxin in peripherial plasma of boars during development, couplation, after administration of human chorionic gonadotropin and after castration. Journal of reproduction and Fertility 107:1-6.

9. Juang HH. 2004. Modulatin of mitochondrial aconitase on the bioenergy of human prostate carcinoma cells. Molecular Genetics and Metabolism 81:244-252.

10. Juang HH. 2004. Cyclic Adenosine 3’,5’- Monosphosphate mediate prolactin regulation of mitochondrial aconitase in human prostate carcinoma cells. Molecular and Cellular Endocrinology 219:141-149.

11. Juang HH. 2004. Nitroprusside stimulates mitochondrial aconitase gene expression through the cyclic adenosine 3’,5’- monosphosphate signal transduction pathway in human prostate carcinoma cells. Prostate 61:92-102.

12. Juang HH. 2004. Modulation of iron on mitochondrial aconitase expression in human prostatic carcinoma cells. Molecular and Cellular Biochemistry 265:185-194.

13. Juang HH,* Hsieh ML, Tsui KH. 2004. Testosterone modulates mitochondrial aconitase in the full-length human androgen receptor-transfected PC-3 prostatic carcinoma cells. Journal of Molecular Endocrinology 33:121-132.

14. Tsui KH, Chang PL, Lin HT, Juang HH.* 2004. Down-regulation of prostate specific antigen promoter by p53 in human prostate cancer cells. Journal of Urology 172:2035-2039.

15. Tsui KH, Wu LL, Chang PL, Hsieh ML, Juang HH.* 2004. Identifying the combination of the transcriptional regulatory sequences on prostate-specific antigen and human glandular kallikrein genes. Journal of Urology 172:2029-2034.

16. Feng TH, Tsui KH, Juang HH.* 2005. Cholesterol modulation of the expression of mitochondrial aconitase in human prostatic carcinoma cells. Chinese Journal of Physiology 48:93-100.

17. Hsieh ML, Juang HH.* 2005. Cell growth effects of triiodothyronine and

expression of thyroid hormone receptor in prostate carcinoma cells. Journal of Andrology 26: 422-428.

18. Tsui KH, Chang PL, Juang HH.* 2006. Zinc blocks gene expression of mitochondrial aconitase in human prostatic carcinoma cells. International Journal of Cancer 118:609-615.

19. Tsui KH, Chang PL, Juang HH. 2006. Maganese antagonizes iron blocking the mitochondrial aconitase expression in human prostate carcinoma cells. Asian Journal of Andrology 8:307-315.

20. Tsui KH, Feng TH, Chang PL, Juang HH.* 2007. Callium deregulate mitochondrial aconitase gene in the human prostate carcinoma cells. Andrology Update 1:304-312.

21. Tsui KH, Chen SM, Wang TM, Juang HH, Cheng CL, Sun GH, Chang PL. 2007. Comparisoms of vided urine cytology, nuclear matrix protein-22 and bladder tumor associated antigen tests for bladder cancer of geriatric male patients in Taiwan. Asian Journal of Andrology 9:711-715.

22. Tsiu KH, Juang HH, Lee TH, Chang PL, Che CL, Yuang B Y-M. 2008. Association of nucleophosmin/B23 with bladder cancer recurrence based on immunohistochemical assessment in clinical samples. Acta Pharmacologica Sinica 29:364-370.

23. Tsui KH, Hsieh WC, Lin MH, Chang PL, Juang HH.* 2008. Triiodothyronine Modulates cell proliferation of human prostatic carcinoma cells by downregulation of the b-cell translocation gene 2. Prostate 68: 610-619.

24. Tsui KH, Feng TH, Chung LC, Chao CH, Chang PL, Juang HH.* 2008. Prostate specific antigen gene expression in androgen insensitive prostate carcinoma subculture cell line. AntiCancer Research 28:1969-1976.

25. Tsui KH, Chang PL, Feng TH, Chung LC, Shu SY, Juang HH.* 2008. Down-regulation of matriptase by overexpression of bikunin attenuates cell invasion in prostate carcinoma cells. AntiCancer Research 28:1977-1984.

26. Tsui KH, Chang PL, Feng TH, Chung LC, Sung HC, Juang HH.* 2008. Evaluating the function of matriptase and N-acetylglucosaminyltransferase V in prostate cancer metastasis. Anticancer Research 28: 1993-2000.

27. Tsui KH, Feng TH, Hsieh WC, Chang PL, Juang HH.* 2008. Expression of interleukin-6 is downregulated by 17-(allylamino)-17-demethoxygeldanamycin in human prostatic carcinoma cells. Acta Pharmacologica Sinica 29:1334-1341.

28. Tsui KH, Feng TH, Lin CM, Chang PL, Juang HH.* 2008. Curcumin blocks the activating of androgen and interlukin-6 on prostate specific antigen expression in human prostatic carcinoma cells. Journal of Andrology 29: 661-668.

29. Pang ST, Hsieh WC, Chuang CK, Chao CH, Weng WH, Juang HH.* 2009.

Thioredoxin-interacting protein: an oxidative-stress related gene is upregulated by glucose in human prostate carcinoma cells. Journal of Molecular Endocrinology 42:205-214.

30. Juang HH, Lin YF, Chang PL, Tsui KH. 2010. Cardiac glycosides reduce prostate specific antigen expression by downregulation of prostate-derived Ets factor. Journal of Urology 84: 2158-2164.

31. Tsui KH, Feng TH, Lin YF, Chang PL, Juang HH.* 2011. p53 downregulates the gene expression of mitochondrial aconitase in human prostate carcinoma cells. Prostate 71:62-70.

32. Tsui KH, Lin YF, ChenYH, Chang PL, Juang HH.* 2011. Mechanisms by which interleukin-6 regulates prostate specific antigen gene expression in prostate LNCaP carcinoma cells. Journal of Andrology 32:383-393.

33. Chung LC, Tsui KH, Feng TH, Lee SL, Chang PL, Juang HH.* 2011. Curcumin provides potential protection against the activation of hypoxia and prolyl 4-hydroxylase inhibitors on prostate-specific antigen expression in human prostate carcinoma cells. Molecular Nutrition and Food Research 55:1666-1676.

34. Chiang KC, Yeh CN, Chen HY, Lee JM, Juang HH, Chen MF, Takano M, Kittaka A, and Chen TC. 2011. 19-Nor-2α-(3-hydroxypropyl)-1α,25-dihydroxyvitamin D3 (MART-10) is a potent cell growth regulator with enhanced chemotherapeutic potency in liver cancer cells. Steroids 76:1513-1519.

35. Chung LC, Tsui KH, Feng TH, Lee SL, Chang PL, Juang HH.* 2012. L-Mimosine blocks cell proliferation via upregulation of B-cell translocation gene 2 and N- myc downstream regulated gene 1 in prostate carcinoma cells American Journal of Physiology Cell Physiology 302:C676-C685.

36. Tsui KH, Chung LC, Feng TH, Chang PL, Juang HH.* 2012. Upregulation of prostate-derived ets factor by luteolin causes inhibition of cell proliferation and cell invasion in prostate carcinoma cells. International Journal of Cancer 130: 2812-2823.

37. Tsui KH, Chang YL, Feng TH, Chang PL, Juang HH.* 2012. Glycoprotein transmembrane nmb: an androgen-downregulated gene attenuates cell invasion and tumorigenesis in prostate carcinoma cells. Prostate 72:1431-1442.

38. Tsui KH, Chang YL, Feng TH, Chung LC, Lee TY, Chang PL, Juang HH.* 2012. Growth differentiation factor-15 upregulates interleukin-6 to promote tumorigenesis of prostate carcinoma PC-3 cells. Journal of Molecular Endocrinology 49:153-163.

39. Chiang KC, Yeh CN, Chen SC, Shen SC, Hsu JT, Yeh TS, Pang JH, Su LJ, Takano M, Kittaka A, Juang HH*, Chen TC*. 2012. MART-10, a new

generation of vitamin D analog, is more potent than 1α,25-dihydroxyvitamin D(3) in inhibiting cell proliferation and inducing apoptosis in ER+ MCF-7 breast cancer cells. Evidence Based Complement Alternative Medicine 2012:e310872.

40. Chiang KC, Yeh CN, Hsu JT, Yeh TS, Jan YY, Wu CT, Chen HY, Jwo SC, Takano M, Kittaka A, Juang HH,* Chen TC.* 2013. Evaluation of the potential therapeutic role of a new generation of vitamin D analog, MART-10, in human pancreatic cancer cells in vitro and in vivo. Cell Cycle 12:1316-1325.

41. Tsui KH, Wang SW, Chung LC, Feng TH, Chen YH, Chang PL, Juang HH.* 2013. Mechanisms by which interleukin-6 attenuates cell invasion and tumorigenesis in human bladder carcinoma cells. BioMed Research International 2013:e791212

42. Yeh CN, Chiang KC, Juang HH, Pang JH S, Yu CS, Lin KJ, Yeh TS, YY Jan 2013. Reappraisal of the therapeutic role of celecoxib in cholangiocarcinoma. PLoS one 8:e69928.

43. Tsui KH, Chung LC, Wang SW, Feng TH, Chang PL, Juang HH.* 2013. Hypoxia upregulates the gene expression of mitochondrial aconitase in prostate carcinoma cells. Journal of Molecular Endocrinology 51:131-141

44. Juang HH, Chung LC, Feng TH, Lee YH, Chang PL, Tsui KH. 2013. Metallothionein 3: an androgen-upregulated gene enhances cell invasion and tumorigenesis of prostate carcinoma cells. Prostate 73:1495-1506. 45. Chiang KC, Yeh CN , Hsu JT , Chen LW , Kuo SF , Sun CC , Huang CC , Pang JHS , Flanagan JN , Takano M, Kittaka A, Juang HH, Yang SW*, Chen TC* . 2013. MART-10, a novel vitamin D analog, inhibits head and neck squamous carcinoma cells growth through cell cycle arrest at G0/G1 with upregulation of p21 and p27 and downregulation of telomerase. Journal of Steroid Biochemistry and Molecular Biology 138:427-434.

46. Lee WC, Lin YH, Hou CP, Chang PL, Chen CL, Juang HH, Tsui KH. 2013. Prostatectomy using different lasers for the treatment of benign prostate hyperplasia in aging males. Clinical Interventions in Aging 8: 1483–1488 47. Chiang KC, Chen SC, Yeh C-N, Pang S JH , Shen SC, Hsu JT, Liu YY, Chen LW, Kuo SF, Takano M, Kittaka A, Sun CC, Juang HH*, Chen TC*. 2014. MART-10, a less calcemic vitamin D analog, is more potent than 1a, 25-dihydroxyvitamin D3 in inhibiting the metastatic potential of MCF-7 breast cancer cells in vitro. Journal of Steroid Biochemistry and Molecular Biology 139:54-60. 48. Chiang KC, Tsui KH, Chung LC, Yeh CN, Chang PL, Chen WT, Juang HH.* 2014. Topoisomerase inhibitors modulate gene expression of B-cell translocation gene 2 and prostate specific antigen in prostate carcinoma cells. PLoS One

9:e89117. 49. Chiang KC, Tsui KH, Chung LC, Yeh CN, Chen WT, Chang PL, Juang HH*. 2014. Celastrol blocks interleukin-6 gene expression via downregulation of NF-κB in prostate carcinoma cells. PLoS One 9:e93151 50. Chiang KC, Yeh CN, Lin KJ, Su LJ, Yen TC, Pang JHS, Kittaka A, Sun CC, Chen MF, Jan YY, Chen TA, Juang HH*, Yeh TS*. 2014. Chemopreventive and Chemotherapeutic Effect of dietary supplementation of vitamin D on cholangiocarcinoma in a chemical-induced animal model. Oncotarget 5:3849-3861.

51. Chiang KC, Tsui KH, Chung LC, Yeh CN, Feng TH, Chen WT, Chang PL, Chiang HY, Juang HH*. 2014. Cisplatin modulates B-cell translocation gene 2 to attenuate cell proliferation of prostate carcinoma cells in both p53-dependent and p53-independent pathways. Scientific Reports 4:5511.

52. Hou CP, Chen CL, Lin YH, Chang PL, Juang HH, Tsui KH*. 2014. Prostatic urethral angle might be a predictor of treatment efficacy of a-blockers in men with lower uringary tract symptoms. Drug Design, Development and Therapy 8:937-943.

53. Yang PS, Hsu YC, Lin YH, Hou CP, Chen CL, Chang PL, Juang HH, Tsui KH*. 2014. The expression sequence tag is an effective method for screening DNA segments that predict urinary bladder transitional cell carcinoma prognosis. OncoTargets and Therapy 7:1777-1781.

54. Shao IH, Lin YH, Hou CP, Juang HH, Chen CL, Chang PL, Tsui KH*. 2014. Risk factors associated with ineligibility of adjuvant cisplatin-based chemotherapy after nephroureterectomy. Drug Design, Development and Therapy 8: 1985–1991.

55. Chiang KC, Yeh CN, Hsu JT, Jan YY, Chen LW, Kuo SF, Takano M, Kittaka Atsushi, Chen TC, Chen WT, Pang JHS, Yeh TS, Juang HH*. 2014. The Vitamin D analog, MART-10, represses metastasis potential via downregulation of epithelial-mesenchymal transition in pancreatic cancer cell. Cancer Letters 354:235-244.

56. Lee JC, Chung LC, Chen YJ, Feng TH, Juang HH*. 2014. N-myc downstream-regulated gene 1 downregulates cell proliferation, invasiveness and tumorigenesis in human oral squamous cell carcinoma. Cancer Letters 355:242-252.

57. Tsui KH, Chung LC, Feng TH, Lee TY, Chang PL, Chen WT, Juang HH*. 2015. Divergent effect of liver X receptor agonists on prostate-specific antigen expression is dependent on androgen receptor in prostate carcinoma cells. Prostate (In Press)

58. Chiang KC, Yeh CN, Chung LC, Feng TH, Sun CC, Chen MF, Jan YY, Chen SC, Yeh TS, Juang HH*. 2015. WNT-1 inducible signaling pathway protein 1 enhances growth and tumorigenesis in human breast cancer. Scientific Reports (In press)

59. Tsui KH, Chung LC, Feng TH, Lee TY, Chang PL, Chen WT, Juang HH*. 2015. Divergent effect of liver X receptor agonists on prostate-specific antigen expression is dependent on androgen receptor in prostate carcinoma cells. Prostate 75:560-672.

60. Lee JC, Chung LC, Chen YJ, Feng TH, Chen WT, Juang HH*. 2015. Upregulation of B-cell translocation gene 2 by epigallocatechin-3-gallate via p38 and ERK signaling blocks cell proliferation in human oral squamous cell carcinoma cells. Cancer Letters. DOI:10.1016/j.canlet.2015.02.034.