蕭永晉

蕭永晉

蕭永晉 (Yung-Chin Hsiao)

職稱：助理教授

研究室名稱：蛋白體轉譯醫學研究室

最高學歷：Ph.D.

學校/國家：中山醫學大學/中華民國

分機號碼：3552

電子郵件帳號：hschin@mail.cgu.edu.tw

個人網頁網址：

研究室現有： 博士後研究員   人

博士班研究生    人

碩士班研究生  人

專任研究助理    人

大學部專生   人

研究方向及研究室特色：

在基因全面解碼的後基因體時代，蛋白質體學分析技術也迎來飛躍的成長。本研究室著重於以先進蛋白質體學定量技術分析生物樣品間的蛋白質差異表現，探討腫瘤的早期診斷、治療標靶、預後評估的相關蛋白質分子。

技術開發: 1) 結合免疫學及蛋白質體學，開發以抗胜肽單株抗體(anti-peptide antibodies)富集樣品中的待測目標胜肽，並利用質譜學多重反應監測技術(Multiple Reaction Monitoring, MRM)，進行多重標靶精準定量，稱之為「免疫-多重反應監測技術」(immuno-MRM)，應用於臨床唾液及血液樣本中準確定量多種低含量的蛋白質標的分子。

2) 建立目標特異性核酸適體(DNA/RNA aptamer, single stranded DNA/RNA specifically bind to target)之體外選殖技術(systematic evolution of ligands by exponential enrichment, SELEX)平台並發展以核酸適體為基礎之癌症檢測平台。

口腔癌研究: 利用MRM及immuno-MRM技術於唾液檢體中驗證能夠區辨口腔癌病人的蛋白質生物標記組(Protein biomarker panel)，建立唾液蛋白質分子的個體差異及日常生理變化之定量化參考資料，進一步驗證生物標記分子在血液及唾液檢體的檢測口腔癌效力，提供實驗數據證實唾液作為常規檢體用於檢測口腔癌的潛力。

腫瘤免疫研究：探討免疫系統在腫瘤發生與惡化的各階段中所扮演的角色，包括: 1) 慢性發炎反應的致癌機轉；2) 腫瘤細胞與免疫細胞間相互作用機制；3) 腫瘤免疫學研究於癌症治療、監測以及抗腫瘤疫苗發展之應用。

最近五年所發表論文：

  1. Liu CC, Yang YH, Hsiao YC, Wang PJ, Liu JC, Liu CH, Hsieh WC, Lin CC, and Yu JS. Rapid and Efficient Enrichment of Snake Venoms from Human Plasma Using a Strong Cation Exchange Tip Column to Improve Snakebite Diagnosis. Toxins (Basel) 2021, 13 (2)Toxins
  2. Liu CC, Wu CJ, Hsiao YC, Yang YH, Liu KL, Huang GJ, Hsieh CH, Chen CK, and Liaw GW. Snake venom proteome of Protobothrops mucrosquamatus in Taiwan: Delaying venom-induced lethality in a rodent model by inhibition of phospholipase A2 activity with varespladib. J Proteomics 2020, 234, 104084.
  3. Lin YH, Wu CC, Su WT, Tseng PC, Hsueh YY, Hsiao YC, Chang KP, Yu JS, and Chuang YJ. Target peptide enrichment microfluidic chip for rapid detection of oral squamous cell carcinoma using stable isotope standards and capture by anti-peptide antibodies. Sensors and Actuators B: Chemical 2020, 322, 128607.
  4. Hsiao YC, Lin SY, Chien KY, Chen SF, Wu CC, Chang YT, Chi LM, Chu LJ, Chiang WF, Chien CY, Chang KP, Chang YS, and Yu JS. An immuno-MALDI mass spectrometry assay for the oral cancer biomarker, matrix metalloproteinase-1, in dried saliva spot samples. Anal Chim Acta 2020, 1100, 118-130.
  5. Chi LM#, Hsiao YC#, Chien KY#, Chen SF, Chuang YN, Lin SY, Wang WS, Chang IY, Yang C, Chu LJ, Chiang WF, Chien CY, Chang YS, Chang KP, and Yu JS. Assessment of candidate biomarkers in paired saliva and plasma samples from oral cancer patients by targeted mass spectrometry. J Proteomics 2020, 211, 103571.
  6. Liu CC, Yu JS, Wang PJ, Hsiao YC, Liu CH, Chen YC, Lai PF, Hsu CP, Fann WC, and Lin CC. Development of sandwich ELISA and lateral flow strip assays for diagnosing clinically significant snakebite in Taiwan. PLoS Negl Trop Dis 2018, 12 (12), e0007014.
  7. Liu CC, Lin CC, Hsiao YC, Wang PJ, and Yu JS. Proteomic characterization of six Taiwanese snake venoms: Identification of species-specific proteins and development of a SISCAPA-MRM assay for cobra venom factors. J Proteomics 2018, 187, 59-68.
  8. Hsiao YC, Chu LJ, Chen YT, Chi LM, Chien KY, Chiang WF, Chang YT, Chen SF, Wang WS, Chuang YN, Lin SY, Chien CY, Chang KP, Chang YS, and Yu JS. Variability Assessment of 90 Salivary Proteins in Intraday and Interday Samples from Healthy Donors by Multiple Reaction Monitoring-Mass Spectrometry. Proteomics Clin Appl 2018, 12 (2)
  9. Hsiao YC, Chu LJ, Chen JT, Yeh TS, and Yu JS. Proteomic profiling of the cancer cell secretome: informing clinical research. Expert Rev Proteomics 2017, 14 (9), 737-756.
  10. Hsiao YC, Chi LM, Chien KY, Chiang WF, Chen SF, Chuang YN, Lin SY, Wu CC, Chang YT, Chu LJ, Chen YT, Chia SL, Chien CY, Chang KP, Chang YS, and Yu JS. Development of a Multiplexed Assay for Oral Cancer Candidate Biomarkers Using Peptide Immunoaffinity Enrichment and Targeted Mass Spectrometry. Mol Cell Proteomics 2017, 16 (10), 1829-1849.
  11. Yu JS#, Chen YT#, Chiang WF#, Hsiao YC#, Chu LJ#, See LC, Wu CS, Tu HT, Chen HW, Chen CC, Liao WC, Chang YT, Wu CC, Lin CY, Liu SY, Chiou ST, Chia SL, Chang KP, Chien CY, Chang SW, Chang CJ, Young JD, Pao CC, Chang YS, and Hartwell LH. Saliva protein biomarkers to detect oral squamous cell carcinoma in a high-risk population in Taiwan. Proc Natl Acad Sci U S A 2016, 113 (41), 11549-11554.
  12. Chen H#, Hsiao YC#, Chiang SF, Wu CC, Lin YT, Liu H, Zhao H, Chen JS, Chang YS, and Yu JS. Quantitative analysis of wild-type and V600E mutant BRAF proteins in colorectal carcinoma using immunoenrichment and targeted mass spectrometry. Anal Chim Acta 2016, 933, 144-155.