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王永樑 (Robert Y.L. Wang)


Lab:Molecular Virology Laboratory


University/Nation:National Taiwan University / Taiwan



Research websitehttps://sites.google.com/site/cgu3691/

Research interests

The goals of Dr. Wang’s research are focused on the interaction of host factors and RNA viruses. Their first project is in the field of developing of yeast as a model host and powerful in vitro assay with purified replicase complexes, together with reverse genetic approaches that make Enterovirus 71 (EV71), Japanese Encephalitis Virus, capable of replicating in yeast, as to study fundamental aspects of viral RNA replication. The second project, Dr. Wang’s laboratory will use proteomic approaches to analyze infection of two cell lineages, RD cells and SF268 cells, with EV71, focusing on the secreted proteins. First, they will identify several secreted proteins which are found only in the EV71-infected cells by the combination using of one dimension electrophoresis and liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). Second, they will perform 2D-electrophoresis followed by MALDI-TOF/TOF in order to validate those proteins. Once they identify the host proteins which were presented in the secreted proteins of EV71-infected cells, they will discuss the results obtained taking into account the proteins previously described in the secretome of virus-infected mammalian cells, proteins present in human plasma and proteins of interest for EV71 pathogenesis. Altogether these data will provide clues for the progress in the understanding of the role of host secretion in disease progression.The specific aims are:

1) Identification of numerous host factors affecting/involving viral replication by using a systems biology approach, including genome-wide screens and proteomics approaches.

2) Dissecting the roles of three RNA-binding host proteins: glyceraldehyde-3-phosphate dehydrogenase (GAPDH), the p68 DEAD-box RNA helicase homolog Dpb2 and translation elongation factor EF-1alpha (Tef1/2p), all of which bind to TBSV RNA and also known to interact with Hepatitis C virus, West Nile virus, dengue 4 virus and hepatitis A virus RNAs, suggesting that host factors might play similar roles in replication of these viruses.

3) Roles of Vacuolar Protein sorting proteins in RNA virus replication.


  1. Xu P, Xu H, Cheng HS, Chan HH, Wang RY*(corresponding author) (2020) MicroRNA 876-5p modulates EV-A71 replication through downregulation of host antiviral factors. Virology Journal; 17:21   (SCI, impact factor: 2.464)
  2. Lai MC, Chen HH, Xu P, Wang RY*(corresponding author) (2020) Translational control of Enterovirus A71 gene expression. Journal of Biomedical Science; 27:22   (SCI, impact factor: 5.203)
  3. Lin CH, Chang L, Chu HW, Lin HJ, Chang PC, Wang RY*(corresponding author), Unnikrishnan B, Mao JY, Chen SY, Huang CC (2019) High amplification of the antiviral activity of curcumin through transformation into carbon quantum dots. Small; DOI: 10.1002/smll.201902641  (SCI, impact factor: 10.856)
  4. Li HY, Tsai MS, Huang CG, Wang RY, Chuang LP, Chen NH, Liu CH, Hsu CM, Cheng WN, Lee LA (2019) Alterations in Alzheimer’s disease-associated gene expression in severe obstructive sleep apnea patients. J. Clin. Med; 8: 1361; DOI: 10.3390/jcm8091361  (SCI, impact factor: 5.688)
  5. Chu HW, Lai CS, Ko JY, Harroun SG, Chuang CI, Wang RY*(corresponding author), Unnikrishnan B, Huang CC (2019) Nanoparticle-based LDI-MS immunoassay for the multiple diagnosis of viral infections. ACS Sensors; DOI: 10.1021/acssensors.9b00054  (SCI, impact factor: 6.944)
  6. Kuo RL, Chen CJ, Wang RY, Huang HI, Lin YH, Tam EH, Tu WJ, Wu SE, Shih SR (2019) Role of Enteroviral RNA-dependent RNA polymerase in regulation of MDA5-mediated beta interferon activation. J. Virology; 93 (10) e00132  (SCI, impact factor: 4.324)
  7. Chen YM, Ou BT, Chen CY, Chan HH, Chen CJ, Wang RY*(corresponding author) (2019) Staufen1 protein participates positively in the viral RNA replication of enterovirus 71. Viruses; 11 142; doi:10.3390/v11020142 (SCI, impact factor: 3.094)
  8. Min N, Vale DS, Wong AA, Tan WH, Chong CY, Chen CJ, Wang RY*(corresponding author), Chu JH* (2018) Circulating Salivary miRNA hsa-miR-221 as Clinically Validated Diagnostic Marker for Hand, Foot, and Mouth Disease in Pediatric Patients. EBioMedicine; 31: 299-306. (SCI, impact factor: 6.680) * Equally contributed authors
  9. Banerjee S, Aponte-Diaz D, Yeager C, Sharma SD, Ning G, Oh HS, Han Q, Umeda M, Hara Y, Wang RY, Cameron C (2018) Hijacking of multiple phospholipid biosynthetic pathways and induction of membrane biogenesis by a picornaviral 3CD protein. PLoS Pathog 14(5): e1007086 (SCI, impact factor: 6.463).
  10. Prabowo BA, Wang RY* (co-first author), Secario MK, Ou PT, Alom A, Liu JJ, Liu KC (2017) Rapid detection and quantification of Enterovirus 71 by a portable surface plasmon resonance biosensor. Biosensors & bioelectronics  DOI: 10.1016/j.bios.2017.01.043 (SCI, impact factor: 9.518) * Equally contributed authors
  11. Lin JJ, Wang RY, Chen CJ, Chiu CC, Liao MH, Wu YJ (2016) Cytotoxicity of 11-epi-Sinulariolide Acetate Isolated from Cultured Soft Corals on HA22T Cells through the Endoplasmic Reticulum Stress Pathway and Mitochondrial Dysfunction. Int. J. Mol. Sci; 17, 1787/doi:10.3390 (SCI, impact factor: 4.183).
  12. Wang RY, Li YJ, Lee WC, Wu HH, Lin CY, Lee CC, Chen YC, Hung CC, Yang CW, Tian YC (2016) The association between polyomavirus BK strains and BKV viruria in liver transplant recipients. Scientific Reports; 6:28491 | DOI: 10.1038 (SCI, impact factor: 4.011)
  13. Wang RY, Weng KF, Huang YC, Chen CJ (2016) Elevated expression of circulating miR876-5p is a specific response to severe EV71 infection. Scientific Reports; 6:24149 | DOI: 10.1038 (SCI, impact factor: 4.011).
  14. Wang RY, Wu YJ, Chen HS, Chen CJ (2016) A KDEL retrieval system for ER-Golgi transport of Japanese Encephalitis viral particles. Viruses; 8 (44); doi: 10.3390/v8020044 (SCI, impact factor: 3.094).
  15. Wang RY, Kuo RL, Yen SM, Chu CH, Wu JY, Huang YC, Chen CJ (2015). Proteome demonstration of alpha-1-acid glycoprotein and alpha-1-antichymotrypsin are candidate biomarkers for diagnosis of Enterovirus 71 infection. The Pediatric Infectious Disease Journal; 34:304-310 (SCI, impact factor: 2.317).
  16. Kuo RL, Lin YH, Wang RY, Hsu CW, Chiu YT, Huang HI, Kao LT, Yu JS, Shih SR, Wu CC. (2015). Proteomics Analysis of EV71-Infected Cells Reveals the Involvement of Host Protein NEDD4L in EV71Replication. J. Proteome Res; 14:1818-1830. (SCI, impact factor: 3.780)
  17. Weng KF, Hung CT, Hsieh PT, Li ML, Chen GW, Kung YA, Huang PN, Kuo RL, Chen LL, Lin JY, Wang RY, Chen SJ, Tang P, Horng JT, Huang HI, Wang, JR, Ojcius DM, Brewer G, Shih SR (2014) A cytoplasmic RNA virus generates functional viral small RNAs and regulates viral IRES activity in mammalian cells. Nucleic Acids Res. 42:12789-12805. (SCI, impact factor: 11.147)
  18. Su C-C, Yang Z-Y, Su J-H, Din Z-H, Wang RY*(corresponding author), Wu Y-J* (2014) 13-Acetoxysarcocrassolide induces apoptosis on human gastric carcinoma cells through mitochondria-related apoptotic pathways: p38/JNK activation and PI3K/AKT suppression. Mar. Drugs; 12: 5295-5315. (SCI, impact factor: 3.772). * Equally contributed authors
  19. Liu Y-C, Kuo R-L, Lin J-Y, Huang P-N, Huang Y, Liu H, Arnold J, Chen S-J, Wang RY, Cameron C, Shih S-R (2014) Cytoplasmic Viral RNA-Dependent RNA Polymerase Disrupts the Intracellular Splicing Machinery by Entering the Nucleus and Interfering with Prp8. PLOS Pathog 10(6): e1004199. doi:10.1371/journal.ppat.1004199. (SCI, impact factor: 6.463)
  20. Huang HI, Chen SK, Wang RY, Shen CR, Cheng YC (2013) Human Foreskin-derived Fibroblast-like Stromal Cells are able to Differentiate into Functional Hepatocyte-like Cells. Cell Biol International; 37(12): 1308-1319. (SCI, impact factor: 2.137)
  21. Wang RY, Kuo RL, Ma WC, Huang HI, Yu JS, Yen SM, Huang CR, Shih SR (2013) Heat Shock Protein-90-beta Facilitates Enterovirus 71 Viral Particles Assembly. Virology; 443: 236-247 (SCI, impact factor: 2.657).
  22. Kuo RL, Kao Li-Ting, Lin SJ, Wang RY, Shih SR (2013) MDA5 plays a crucial role in Enterovirus 71 RNA mediated IRF3 activation. PLoS ONE 8(5): e63431. doi:10.1371/journal.pone.0063431(SCI, impact factor: 2.776)
  23. Neoh CA*, Wang RY* (co-first author), Din ZH, Su JH, Chen YK, Tsai FJ, Weng SH, Wu YJ (2012) Induction of Apoptosis by Sinulariolide from Soft Coral through Mitochondrial-Related and p38MAPK Pathways on Human Bladder Carcinoma Cells. Mar. Drugs; 10: 2893-2911. (SCI, impact factor: 3.772) * Equally contributed authors
  24. Huang CR, Wang RY, Hsu SC, Lo SJ (2012) Lysine-71 in the Large Delta Antigen of Hepatitis Delta Virus Clade 3 Modulates its Localization and Secretion. Virus Res; 170: 75-84. (SCI, impact factor: 2.736)
  25. Liu CI*, Wang RY* (co-first author), Lin JJ, Su JH, Chiu CC, Chen JC, Chen JY, Wu YJ (2012) Proteomic Profiling of the 11-Dehydrosinulariolide-treated Oral Carcinoma Cells Ca9-22: effects on the cell apoptosis through Mitochondrial-related and ER stress pathway. J. Proteomics; 75:5578-5589. (SCI, impact factor: 3.537) * Equally contributed authors
  26. Wang RY, Kui Li. (2012) Host factors in the replication of positive-strand RNA viruses. Chang Gung Med J; 35: 111-124.
  27. Li YJ, Wu HH, Weng CH, Chen YC, Hung CC, Yang CW, Wang RY, Sakamoto N, Tian YC. (2012) Cyclophilin A and nuclear factor of activated T cells are essential in cyclosporin A-mediated suppression of polyomavirus BK replication. American J Transplantation; doi:10.1111/j.1600-6143.2012.04116.x. (SCI, impact factor: 7.163)
  28. Hung CY, Tsai MC, Wu YP, Wang RY*. (2011)  Identification of heat shock protein 90 beta in JEV-induced secretion proteins. Journal of General Virology; 92:2803-2809. (SCI, impact factor: 2.809) *Corresponding author
  29. Prasanth KR, Huang YW, Liou MR, Wang RY, Hu CC, Tsai CH, Meng M, Lin NS, Hsu YH. (2011)  Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) negatively regulates the replication of Bamboo mosaic virus and its associated satellite RNA. Journal of Virology; 85: 8829-8840. (SCI, impact factor: 4.324)
  30. Nagy PD, Wang RY, Pogany J, Hafren A, Makinen K.  (2011) Emerging picture of host chaperone and cyclophilin roles in RNA virus replication. Virology; 411:374-382. (SCI, impact factor: 2.657)
  31. Wang RY, Huang YR,  Chong KM, Hung CY, Ke ZL, Chang RY. (2011DnaJ homolog Hdj2 Facilitates Japanese Encephalitis Virus Replication. Virology J; 8:471. (SCI, impact factor: 2.464)
  32. Wu YP, Chang CM, Schuyler S, Hung CY, Tsai MC, Wang RY*. (2011) Japanese Encephalitis Virus Co-opts the ER-Stress Response Protein GRP78 for Viral Infectivity. Virology J; 8:128. (SCI, impact factor: 2.464) *Corresponding author.
  33. Wang RY, Stork J, Pogany J, Nagy PD. (2009) A temperature sensitive mutant of heat shock protein 70 reveals an essential role during the early steps of tombusvirus replication. Virology; 394:28-38. (SCI, impact factor: 2.657).
  34. Wang RY, Stork J, Nagy PD (2009). A key role for heat shock protein 70 in localization and insertion of the tombusvirus replication proteins to intracellular membranes. J. Virology; 83:3276-3287 (SCI, impact factor: 4.324)
  35. Wang RY, Nagy PD (2008). Tomato bushy stunt virus Co-Opts the RNA-Binding Function of a Host Metabolic Enzyme for Viral Genomic RNA Synthesis. Cell Host&Microbe; 3:178-187. (SCI, impact factor: 15.753).
  36. Wang RY, Shen CN, Tosh D, Lin MH, Shih C (2005). Hepatocyte-like cells transdifferentiated from pancreatic origin can support replication of hepatitis B virus. J. Virology;79:13116-13128. (SCI, impact factor: 4.324).
  37. Chua PK, Wang RY, Suk FM, Lin MH, Masuda T, and Shih C (2005). A Naturally Occurring Mutation (L77R) of Hepatitis B Virus Small S Envelope Gene Results in Low-Level Virion Secretion and Can be Suppressed by Another Natural Envelope Mutation (W74L). J. Virology;79:13483-13496. (SCI, impact factor: 4.324).
  38. Wang YL, Wu CY, Chang CT, Sung HY (2003). Invertase inhibitors from sweet potato (Ipomoea Batatas): purification and biochemical characterization. J. Agric. Food Chem;51:4804-4809. (SCI, impact factor: 3.751)
  39. Wang YL, Lee YH, Chen CC, Chang CT, Sung HY (1998). Purification and characterization of soluble acid invertase from yam tubers. J. Chinese Agric. Chem. Soc;36: 464-472.
  40. Fu RH*, Wang YL* ,Sung HY (2002). Biochemical and molecular biological studies of plant invertases. Food Sci. Agric. Chem.;4:1-7 (SCI, impact factor: 3.751) (*Equally  contribution).
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