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陳怡原

                            

 Yi-Ywan Margaret Chen (陳怡原)

Appointment: Professor

LabBacterial Genetics Research Laboratory

Education: Ph.D.

University/NationUniv. of Texas HSC at San Antonio, USA

Tel: (03)2118800 ext. 3352

E-mail: mchen@mail.cgu.edu.tw

Research website:

Laboratory personnel:

Postdoctoral fellow: 0; Ph.D. student: 0; Master student: 2; Res. Assistant: 3; Undergraduate: 5

Research interests:

Dental plaque is one of the most successful biofilms in the human body and is essential for the development of chronic infections such as dental caries and periodontal diseases. Studies in recent years have demonstrated a clear link between periodontal diseases and systemic conditions such as coronary heart disease and stroke, and a higher risk of preterm low birth-weight babies. My research interests primarily focus on the molecular mechanisms regulating the physiology and virulence expression of oral bacteria in response to environmental conditions, and the development of therapeutic strategies for controlling oral infections. We are particularly interested in the process of biofilm formation and how the biofilm lifestyle affects the physiology and virulence capacity of the oral streptococci. To accomplish this, a variety of microbiological, biochemical, and molecular biology technologies are employed. These include the use of continuous chemostat culture system to tightly control growth parameters, coupled with the use of genetically-engineered bacteria and gene fusion technology, which allows for a detailed analysis of gene expression in response to specific stimuli. Systemic approaches including transcriptomic and proteomic analyses are also employed. The first specific project focuses on the molecular and functional analyses of the twitching motility of Streptococcus sanguinis, the only streptococcal species expressing this phenotype. The second specific project focuses on the differential regulation of virulence genes expression in Streptococcus sanguinis and Streptococcus salivairus in response to environmental cues.

Recent Publications (2015-2020)

  1. Yi-Ywan M. Chen* Pei-Hua Tsai, Zong-Sian Ye, Yu-Wen Huang, Hui-Ru Shieh, Chia-Hua Wu, Yu-Juan Lin, James H. Miller, Jacqueline Abranches, Cheng-Hsun Chiu. 2020. Functional analysis of the collagen binding proteins of Streptococcus parasanguinis FW213. mSphere. 5:e00863-20.
  2. Yi-Ywan. M. Chen*, Pei-Shan Lu, Pei-Hua Tsai, Cheng-Hsun Chiu. 2020. Functional analysis of a fibronectin binding protein of Streptococcus parasanguinis FW213. Curr Microbilol. 77(11):3430-34.
  3. Yu-Wen Chen, Mei-Zi Huang, Chyi-Liang Chen, Chieh-Ying Kuo, Chia-Yu Yang, Chuan Chiang-Ni, Yi-Ywan. M. Chen, Chia-Ming Hsieh, Hui-Yu Wu, Ming-Ling Kuo, Cheng-Hsun Chiu and Chih-Ho Lai. 2020. PM2.5 impairs macrophage functions to exacerbate pneumococcus-induced pulmonary pathogenesis. Part Fibre Toxicol. 17:37.
  4. Yi-Ywan. M. Chen*, Yi-Chien Chiang, Tzu-Ying Tseng, Hui-Yu Wu, Yueh-Ying Chen, Chia-Hua Wu, Cheng-Hsun Chiu. 2019. Molecular and functional analysis of the type IV pilus gene cluster in Streptococcus sanguinis SK36. Appl Environ Microbiol. 85(6):e02788-18.
  5. J. Geng, S-C. Huang, Y-Y. Chen, C-H Chiu, S. Hu, and Yi-Ywan. M. Chen*. 2018. Impact of growth pH and glucose concentrations on the CodY regulatory network in Streptococcus salivarius. BMC Genomics 19:386.
  6. W-C. Chou, S-C. Huang, C-H. Chiu, Yi-Ywan. M. Chen*. 2017. YMC-2011, a temperate phage of Streptococcus salivarius 57.I. Appl. Environ. Microbiol. 83(6): e03186-16.
  7. Yi-Ywan. M. Chen*, Y-Y. Chen, J-L. Hung, P-M. Chen, J-S. Chia. 2016. The GlnR Regulon in Streptococcus mutans is differentially regulated by GlnR and PmrA. PLoS ONE 11(7):e0159599.
  8. Yi-Ywan. M. Chen*, Y-Y. Chen, J-L. Hung, P-M. Chen, J-S. Chia. 2016. The GlnR Regulon in Streptococcus mutans is differentially regulated by GlnR and PmrA. PLoS ONE 11(7): 0159599.
  9. S-C. Huang, and. Yi-Ywan. M. Chen*. 2016. Role of VicRKX and GlnR in pH dependent regulation of the Streptococcus salivarius 57.I urease operon. mSphere 1:e00033-15.
  10. C-J. Chang, C-S. Lin, C-C. Lu, J. Martel, Y-F. Ko, D. M. Ojcius, S-F. Tseng, T-R. Wu, Yi-Ywan. M. Chen, J. D. Young and H-C. Lai. 2015. Ganoderma lucidum reduces obesity in mice by modulating the composition of the gut microbiota. 2015, Nat. Commun. 6:7489
  11. T-W. Chen, R-C. Gan, Y-F. Chang, W-C. Liao,T. H. Wu, C-C. Lee,P-J. Huang, C-Y. Lee, Yi-Ywan. M. Chen, C-H. Chiu, and P. Tang. 2015. Is the whole greater than the sum of its parts? De novo assembly strategies for bacterial genomes based on paired-end sequencing. BMG Genomics 16:648.
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