The field of chromatin biology/epigenomics has been a fascinating area of research inquiry in the last decade or so mainly due to the isolation and identification of a myriad of chromatin structure modulators. Through remodeling (or further packaging) or modification of the nucleosomal assembly, or establishing particular histone code or DNA methylation pattern, these types of “epigenetic regulator” activities have proven intrinsically vital to the control of gene expression and DNA structure maintenance, and ultimately, cell physiology. My research is focused mainly on several novel chromatin-associated factors, all of which have distinct yet largely uncharacterized epigenetic roles during different chromatin-based processes.
The field of chromatin biology/functional epigenomics focuses on the functional integration of many signaling pathways and different chromatin-associated enzymatic or structural factors. Therefore, to thoroughly characterize the functions of our target chromatin modifiers, we are undertaking a multi-disciplinary scheme entailing various tools and methods:
A. An integrated, functional genomic-based approach based on powerful methods such as ChIP-on-chip, microarray, TF-TF array, and interactomic/proteomic analyses.
B. A cell biology/developmental biology approach, including techniques such as immunocytochemistry, siRNA-mediated gene knock down, cell cycle profiling, and mouse genetics.
1. Liu H, Tan BC (co-first author), Tseng KH, Chuang CP, Yeh CW, Chen KD, Lee SC, and Yung BYM*. NPM acts as a novel AP-2-binding transcriptional corepressor during cell differentiation. EMBO Rep. 2007 Apr; 8 (4): 394-400.
2. Birch JL, Tan BC (co-first author), Panov KI, Panova TB, Andersen JS, Owen-Hughes TA, Russell J, Lee SC, Zomerdijk JCBM*. FACT facilitates chromatin transcription by RNA polymerases I and III. EMBO J. 2009 Apr 8; 28: 854-865.
3. Lin HC, Wu JT, Tan BC, and Chien CT*. Cul4 and DDB1 regulate Orc2 localization, BrdU incorporation and Dup stability during gene amplification in Drosophila follicle cells. J Cell Sci. 2009 Jul 15; 122: 2393-2401.
4. Chen YJ, Tan BC, Cheng YY, Chen JS, and Lee SC*. Differential regulation of CHOP translation by phosphorylated eIF4E under stress condition. Nucleic Acids Res. 2010 Jan; 38(3): 764-77.
5. Tan BC*, Liu H, Lin CL, Lee SC*. Functional cooperation between FACT and MCM is coordinated with cell cycle and differential complex formation. J Biomed Sci. 2010 Feb 16; 17(1):11.
6. Lin CY, Tan BC* (co-first author), Liu H, Shih CJ, Chien KY, Lin CL, and Yung BYM*. Dephosphorylation of nucleophosmin by PP1 facilitates pRB binding and consequent E2F1-dependent DNA repair. Mol Bio Cell. 2010 Dec 15; 21(24): 4409-4417.
7. Hsieh CL, Lin CL, Liu H, Chang YJ, Shih CJ, Zhong CZ, Lee SC*, and Tan BC*. WDHD1 modulates the post-transcriptional step of the centromeric silencing pathway. Nucleic Acids Res. 2011 May 10; 39(10): 4048-4062.
8. Peng Z, Cheng Y, Tan BC (co-frist author), Kang L, Tian Z, Zhu Y, Zhang W, Liang Y, Hu X, Tan X, Guo J, Dong Z, Liang Y, Bao L, and Wang J*. Comprehensive analysis of RNA-Seq data reveals extensive RNA editing in a human transcriptome. Nat. Biotechnol. 2012 March; 30(3): 253-260.
9. Yang CC, Liu H, Chen SL, Wang TH, Hsieh CL, Huang Y, Chen SJ, Chen HC, Yung BYM*, Tan BC*. 2012. Epigenetic silencing of myogenic gene program by Myb-binding protein 1a suppresses myogenesis. EMBO J. 2012; accepted.