The field of
chromatin biology/epigenomics has been a
fascinating area of research inquiry in the last decade or so mainly due to
the isolation and identification of a myriad of chromatin structure
modulators. Through remodeling (or further packaging) or modification of the nucleosomal assembly, or establishing particular histone
code or DNA methylation pattern, these types of “epigenetic regulator”
activities have proven intrinsically vital to the control of gene expression
and DNA structure maintenance, and ultimately, cell physiology. My research
is focused mainly on several novel chromatin-associated factors, all of which
have distinct yet largely uncharacterized epigenetic roles during different
The field of chromatin biology/functional epigenomics
focuses on the functional integration of many signaling pathways and
different chromatin-associated enzymatic or structural factors. Therefore, to
thoroughly characterize the functions of our target chromatin modifiers, we
are undertaking a multi-disciplinary scheme entailing various tools and
A. An integrated, functional genomic-based approach
based on powerful methods such as ChIP-on-chip,
microarray, TF-TF array, and interactomic/proteomic
B. A cell biology/developmental biology approach,
including techniques such as immunocytochemistry, siRNA-mediated
gene knock down, cell cycle profiling, and mouse genetics.
1. Liu H, Tan
BC (co-first author), Tseng KH, Chuang CP, Yeh CW, Chen KD, Lee SC, and Yung BYM*. NPM acts as a
novel AP-2-binding transcriptional corepressor
during cell differentiation. EMBO Rep. 2007 Apr; 8
2. Birch JL, Tan BC (co-first
author), Panov KI, Panova
TB, Andersen JS, Owen-Hughes TA, Russell J, Lee SC, Zomerdijk
JCBM*. FACT facilitates chromatin transcription by RNA polymerases I and III.
EMBO J. 2009 Apr 8; 28: 854-865.
3. Lin HC, Wu JT, Tan BC, and Chien CT*. Cul4 and DDB1 regulate Orc2 localization, BrdU incorporation and Dup stability during gene
amplification in Drosophila follicle cells. J Cell Sci. 2009
Jul 15; 122: 2393-2401.
4. Chen YJ, Tan BC, Cheng YY,
Chen JS, and Lee SC*. Differential regulation of CHOP translation by
phosphorylated eIF4E under stress condition. Nucleic Acids
Res. 2010 Jan; 38(3): 764-77.
5. Tan BC*, Liu H, Lin CL, Lee SC*.
Functional cooperation between FACT and MCM is coordinated with cell cycle
and differential complex formation. J Biomed Sci. 2010
Feb 16; 17(1):11.
6. Lin CY, Tan BC* (co-first
author), Liu H, Shih CJ, Chien KY, Lin CL, and Yung
BYM*. Dephosphorylation of nucleophosmin
by PP1 facilitates pRB binding and consequent
E2F1-dependent DNA repair. Mol
Bio Cell. 2010 Dec 15; 21(24): 4409-4417.
7. Hsieh CL, Lin CL, Liu H, Chang YJ, Shih CJ, Zhong
CZ, Lee SC*, and Tan BC*. WDHD1
modulates the post-transcriptional step of the centromeric
silencing pathway. Nucleic Acids Res.
2011 May 10; 39(10): 4048-4062.
8. Peng Z, Cheng Y, Tan BC
(co-frist author), Kang L, Tian
Z, Zhu Y, Zhang W, Liang Y, Hu X, Tan X, Guo J,
Dong Z, Liang Y, Bao L, and Wang J*. Comprehensive
analysis of RNA-Seq data reveals extensive RNA
editing in a human transcriptome. Nat.
Biotechnol. 2012 March; 30(3):
9. Yang CC, Liu H, Chen SL, Wang TH, Hsieh CL, Huang Y, Chen SJ, Chen HC,
Yung BYM*, Tan BC*. 2012.
Epigenetic silencing of myogenic gene program by Myb-binding
protein 1a suppresses myogenesis. EMBO
J. 2012; accepted.