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王永樑

王永樑(Robert Y.-L. Wang)

職稱：教授

研究室名稱：分子病毒研究室 (第一醫學大樓十樓B區)

最高學歷：Ph.D.

學校/國家：國立台灣大學微生物與生化學所

分機號碼：3691

電子郵件帳號： yuwang@mail.cgu.edu.tw

個人網頁網址：https://sites.google.com/site/cgu3691/

博士後研究員  人

博士班研究生 人

碩士班研究生  人

專任研究助理  人

大學部專題生  人

研究方向及研究室特色：

 實驗室研究主題：腸病毒 71 型及日本腦炎病毒之病毒複製和宿主之間的相互作用機制。

我們實驗室主要從事高深的病毒與寄主互相作用的研究。目前實驗室執行國科會計畫有兩大方向：

(一) 嘗試將兩種正股 RNA 病毒 (腸病毒 71 型 Enterovirus 71，EV71及 日本腦炎, Japanese Encephalitis Virus，JEV)，轉型進入酵母菌中，測試其是否可以利用酵母菌為宿主進行複製；進一步利用這種最簡單的真核生物，進行病毒與寄主蛋白的互動機制研究。這項研究策略是基於過去我在美國肯塔基大學病理學部門進行研究工作。我使用tombusvirus(一種感染於植物的 positive stranded RNA病毒)作為一個模型。加上以Yeast為宿主進行實驗，成功地證明Glyceraldehyde-3-phosphate　dehydrogenas (GAPDH)，一種著名的代謝酵素，能透過它的選擇性結合不同股　RNA　的能力提升RNA 病毒的複製。 這重要發現將領導病毒學家理解病毒能利用寄主細胞內原有的蛋白質,提供本身複製和演化的一項工具。 這也提供研究對抗病毒及病毒傳染病作戰的一個新的策略及線索。我的這項研究工作也發表於 Cell Host&Microbe , 這是全世界最好的研究病毒及寄主互相作用雜誌之一。

(二) 利用蛋白質體學方法，分析受到腸病毒 71 型及日本腦炎病毒感染後細胞之外分泌蛋白的組成，並且探討其可能影響病毒於體內複製，體外感染的機制。我的主要研究方向如下：

  1. 利用系統生物學的方式，包括基因組及蛋白質體的篩選方式，尋找可能參與/影響 RNA 病毒複製的宿主蛋白。進而研究這些蛋白對於病毒複製的角色與實際參與的功能。
  2. 分析及功能性闡述三種 RNA 結合性宿主蛋白於 RNA 病毒複製過程中參與的角色。包括：Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), p68 DEAD-box RNA helicase homolog Dp2 以及蛋白轉譯延長因子 (EF-1 alpha)。
  3. 負責液泡性蛋白分煉之功能性蛋白對於 RNA 病毒複製的角色。

  Published Papers:

  1. Cheng CH, Peng YC, Lin SM, Yatsuda H,  Liu SH, Liu SJ,  Kuo CY, Wang RY* (corresponding author) (2022) Measurements of anti-SARS-CoV-2 antibody levels after vaccination using a SH-SAW biosensor. Biosensors. 12, 599. (SCI, impact factor: 5.743).
  2. Chen HH, Lin CJ, Anand A, Lin HJ, Lin HY, Mao JY, Wang PH, Tseng YJ, Tzou WS, Huang CC, Wang RY* (corresponding author) (2022) Development of antiviral carbon quantum dots that target the Japanese encephalitis virus envelope protein. J Biol Chem. 298(6): 101957. (SCI, impact factor: 5.486).
  3. Liu S, Dong Y, Wang Y, Hu P, Wang J, Wang RY* (corresponding author) (2021) Pristimerin exerts antitumor activity against MDA-MB-231 triple-negative breast cancer cells by reversing of epithelial-mesenchymal transition via downregulation of integrin b3. Biomedical J. 44: S84-S92. (SCI, impact factor: 7.892)
  4. Peng YC, Cheng CH, Yatsuda H, Liu SH, Liu SJ, Kogai T, Kuo CY, Wang RY*(corresponding author) (2021) A Novel Rapid Test to Detect Anti-SARS-CoV-2 N Protein IgG Based on Shear Horizontal Surface Acoustic Wave (SH-SAW). Diagnostics 11:1838 (SCI, impact factor: 3.992)
  5. Li YJ, Wu HH, Chen CH, Wang HH, Chiang YJ, Hsu HH, Pang ST, Wang RY, Tian YC (2021) High Incidence and Early Onset of Urinary Tract Cancers in Patients with BK Polyomavirus Associated Nephropathy. Viruses; 13: 476 (SCI, impact factor: 5.818)
  6. Liao T, Wang Xi, Donolato M, Cruz MM, Balmaseda A, Harris E, Wang RY*(corresponding author) (2020) Evaluation of ViroTrack Sero Zika IgG/IgM, a new rapid and quantitative Zika serological diagnostic assay. Diagnostics 10:372; doi:10.3390 (SCI, impact factor: 3.992)
  7. Xu P, Xu H, Cheng HS, Chan HH, Wang RY*(corresponding author) (2020) MicroRNA 876-5p modulates EV-A71 replication through downregulation of host antiviral factors. Virology Journal; 17:21   (SCI, impact factor: 5.922)
  8. Lai MC, Chen HH, Xu P, Wang RY*(corresponding author) (2020) Translational control of Enterovirus A71 gene expression. Journal of Biomedical Science; 27:22   (SCI, impact factor: 12.771)
  9. Lin CH, Chang L, Chu HW, Lin HJ, Chang PC, Wang RY*(corresponding author), Unnikrishnan B, Mao JY, Chen SY, Huang CC (2019) High amplification of the antiviral activity of curcumin through transformation into carbon quantum dots. Small; DOI: 10.1002/smll.201902641  (SCI, impact factor: 15.153)
  10. Li HY, Tsai MS, Huang CG, Wang RY, Chuang LP, Chen NH, Liu CH, Hsu CM, Cheng WN, Lee LA (2019) Alterations in Alzheimer’s disease-associated gene expression in severe obstructive sleep apnea patients. J. Clin. Med; 8: 1361; DOI: 10.3390/jcm8091361  (SCI, impact factor: 4.964)
  11. Chu HW, Lai CS, Ko JY, Harroun SG, Chuang CI, Wang RY*(corresponding author), Unnikrishnan B, Huang CC (2019) Nanoparticle-based LDI-MS immunoassay for the multiple diagnosis of viral infections. ACS Sensors; DOI: 10.1021/acssensors.9b00054  (SCI, impact factor: 9.618)
  12. Kuo RL, Chen CJ, Wang RY, Huang HI, Lin YH, Tam EH, Tu WJ, Wu SE, Shih SR (2019) Role of Enteroviral RNA-dependent RNA polymerase in regulation of MDA5-mediated beta interferon activation. J. Virology; 93 (10) e00132  (SCI, impact factor: 6.549)
  13. Chen YM, Ou BT, Chen CY, Chan HH, Chen CJ, Wang RY*(corresponding author) (2019) Staufen1 protein participates positively in the viral RNA replication of enterovirus 71. Viruses; 11 142; doi:10.3390/v11020142 (SCI, impact factor: 5.818)
  14. Min N, Vale DS, Wong AA, Tan WH, Chong CY, Chen CJ, Wang RY*(corresponding author), Chu JH* (2018) Circulating Salivary miRNA hsa-miR-221 as Clinically Validated Diagnostic Marker for Hand, Foot, and Mouth Disease in Pediatric Patients. EBioMedicine; 31: 299-306. (SCI, impact factor: 11.205) * Equally contributed authors
  15. Banerjee S, Aponte-Diaz D, Yeager C, Sharma SD, Ning G, Oh HS, Han Q, Umeda M, Hara Y, Wang RY, Cameron C (2018) Hijacking of multiple phospholipid biosynthetic pathways and induction of membrane biogenesis by a picornaviral 3CD protein. PLoS Pathog 14(5): e1007086 (SCI, impact factor: 7.464).
  16. Prabowo BA, Wang RY* (co-first author), Secario MK, Ou PT, Alom A, Liu JJ, Liu KC (2017) Rapid detection and quantification of Enterovirus 71 by a portable surface plasmon resonance biosensor. Biosensors & bioelectronics  DOI: 10.1016/j.bios.2017.01.043 (SCI, impact factor: 12.545) * Equally contributed authors
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