洪錦堂 (Jim-Tong Horng)

職稱：教授

研究室名稱：抗新冠病毒藥物研發實驗室

最高學歷：Ph.D.

學校/國家：劍橋大學/英國

分機號碼：5156

電子郵件帳號： jimtong@mail.cgu.edu.tw

個人網頁網址：https://horng0866.wixsite.com/jimtong

研究室現有： 博士後研究員3人

博士班研究生1人

碩士班研究生1人

專任研究助理4人

大學部專題生5人

研究方向及研究室特色：(2021.4更新)

研究題目: Drug discovery against RNA viruses 

研究方向:

1.我們實驗室從事抗病毒中藥與小分子藥物的開發，希望從臨床中藥複方或單味中藥，來開發與篩選具抗流行性感冒病毒、新冠病毒與腸病毒的抑制藥物，並研究其機轉，以因應可能爆發之疫情。

2. 利用定量蛋白質體學技術/高通量次世代定序，鑑定藥物之靶標蛋白質，透過miRNA/lncRNA表現差異，誘發訊號傳遞，了解宿主-病毒的相互調控，並研究其相關的抗病毒機制。

最近五年所發表論文：(#first author, *corresponding author, 2016-2021)

1. Tang, W. F., Huang, R. T., Chien, K. Y., Huang, J. Y., Lau, K. S., Jheng, J. R., Chiu, C. H., Wu, T. Y., Chen, C. Y., and Horng*, J. T. (2016) Host MicroRNA miR-197 Plays a Negative Regulatory Role in the Enterovirus 71 Infectious Cycle by Targeting the RAN Protein. Journal of virology 90, 1424-1438

2. Jheng, J. R., Wang, S. C., Jheng, C. R., and Horng*, J. T. (2016) Enterovirus 71 induces dsRNA/PKR-dependent cytoplasmic redistribution of GRP78/BiP to promote viral replication. Emerg Microbes Infect 5, e23

3. Ho, J. Y., Chern, J. H., Hsieh, C. F., Liu, S. T., Liu, C. J., Wang, Y. S., Kuo, T. W., Hsu, S. J., Yeh, T. K., Shih, S. R., Hsieh, P. W., Chiu, C. H., and Horng*, J. T. (2016) In vitro and in vivo studies of a potent capsid-binding inhibitor of enterovirus 71. The Journal of antimicrobial chemotherapy 71, 1922-1932

4. Lin, T. J., Lin, C. F., Chiu, C. H., Lee, M. C., and Horng*, J. T. (2016) Inhibition of endosomal fusion activity of influenza virus by Rheum tanguticum (da-huang). Sci Rep 6, 27768

5. Hsieh, C. F., Chen, Y. L., Lin, C. F., Ho, J. Y., Huang, C. H., Chiu, C. H., Hsieh, P. W., and Horng*, J. T. (2016) An extract from Taxodium distichum targets hemagglutinin- and neuraminidase-related activities of influenza virus in vitro. Sci Rep 6, 36015

6. Chang, Y. H., Lau, K. S., Kuo, R. L., and Horng*, J. T. (2017) dsRNA Binding Domain of PKR Is Proteolytically Released by Enterovirus A71 to Facilitate Viral Replication. Front Cell Infect Microbiol 7, 284

7. Chen, S. G., Cheng, M. L., Chen, K. H., Horng, J. T., Liu, C. C., Wang, S. M., Sakurai, H., Leu, Y. L., Wang, S. D., and Ho, H. Y. (2017) Antiviral activities of Schizonepeta tenuifolia Briq. against enterovirus 71 in vitro and in vivo. Sci Rep 7, 935

8. Jheng, J. R., Lau, K. S., Lan, Y. W., and Horng, J. T. (2018) A novel role of ER stress signal transducer ATF6 in regulating enterovirus A71 viral protein stability. Journal of biomedical science 25, 9

9. Huang, C. G., Lee, L. A., Wu, Y. C., Hsiao, M. J., Horng, J. T., Kuo, R. L., Huang, C. H., Lin, Y. C., Tsao, K. C., Chen, M. C., Chen, T. C., and Shih, S. R. (2018) A pilot study on primary cultures of human respiratory tract epithelial cells to predict patients' responses to H7N9 infection. Oncotarget 9, 14492-14508

10. Sethy, B., Hsieh, C. F., Yeh, C., Horng, J. T., and Hsieh, P. W. (2018) Design, synthesis & structure-activity relationships of a new class of antihuman enterovirus D68 & A71 agents. Future Med Chem 10, 1333-1347

11. Tang, W. F., Huang, R. T., Chien, K. Y., Tang, P., and Horng*, J. T. (2018) Large-Scale Proteomic Identification of Targets of Cellular miR-197 Downregulated by Enterovirus A71. Journal of proteome research

12. Sethy, B., Hsieh, C. F., Lin, T. J., Hu, P. Y., Chen, Y. L., Lin, C. Y., Tseng, S. N., Horng*, J. T., and Hsieh*, P. W. (2019) Design, Synthesis, and Biological Evaluation of Itaconic Acid Derivatives as Potential Anti-Influenza Agents. Journal of medicinal chemistry 62, 2390-2403

13. Hsieh, C.F., Jheng, J.R., Lin, G.H., Chen, Y.L., Ho, J.Y., Liu, C.J., Hsu, K.Y., Chen, Y.S., Chan, Y.F., Yu, H.M., Hsieh, P.W., Chern, J.H., Horng*, J.T., 2020. Rosmarinic acid exhibits broad anti-enterovirus A71 activity by inhibiting the interaction between the five-fold axis of capsid VP1 and cognate sulfated receptors. Emerg Microbes Infect 9, 1194-1205.

執行中的計畫:

Ongoing Research Support

MOST 109-2320-B-182 -026 -MY3      Horng (PI)      2020/08/01  ~   2023/07/31

衣康酸衍生物抑制流感病毒核酸蛋白出核的分子機轉研究

MOST 109-2634-F-182-001     Horng (co-PI)         2021/03/01  ~  2022/02/28

高教深根與特色中心計畫The Featured Areas Research Center Program within the framework of the Higher Education  Sprout Project by the Ministry of Education (MOE) in Taiwan and the Ministry of Science and Technology.

MOST 109-2327-B-182 -003 -       Horng (PI)         2020/07/01  ~   2021/05/31

以新冠病毒RNA聚合酶為標的進行藥物開發